AdipoQ is a novel adipose-specific gene dysregulated in obesity
AdipoQ is a novel adipose-specific gene dysregulated in obesity
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DOI：
10.1074/jbc.271.18.10697
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发表时间：
1996-05-03
影响因子：
4.8
通讯作者：
Spiegelman, BM
中科院分区：
文献类型：
--
作者：
Hu, E;Liang, P;Spiegelman, BM
脂肪分化伴随着细胞形态的改变、细胞内脂质的大量积累和特定基因表达程序的激活。利用mRNA差异显示技术，我们分离了一个新的脂肪cDNA，命名为adipoQ。adipoQ cDNA编码247个氨基酸的多肽，在氨基末端具有分泌信号序列、胶原区域（Gly-X-Y重复）和球状结构域。adipoQ的球状结构域与补体因子Clq、胶原α 1（X）和脑特异性因子小脑蛋白的亚基具有显著的同源性。adipoQ的表达对小鼠和大鼠的脂肪组织都具有高度特异性。adipoQ的表达仅在成熟脂肪细胞中观察到，因为脂肪组织的基质-血管部分不含adipoQ mRNA。在培养的3 T3-F442 A和3 T3-L1前脂肪细胞中，脂肪酸诱导的分化显著增加了adipoQ的表达水平。此外，在来自肥胖小鼠和人的脂肪组织中，adipoQ mRNA的表达显著降低。而这种多肽的生物学功能目前尚不清楚，组织特异性表达的一个假定的分泌蛋白表明，这种因素可能作为一种新的信号分子脂肪组织。
Adipose differentiation is accompanied by changes in cellular morphology, a dramatic accumulation of intracellular lipid and activation of a specific program of gene expression, Using an mRNA differential display technique, we have isolated a novel adipose cDNA, termed adipoQ. The adipoQ cDNA encodes a polypeptide of 247 amino acids with a secretory signal sequence at the amino terminus, a collagenous region (Gly-X-Y repeats), and a globular domain. The globular domain of adipoQ shares significant homology with subunits of complement factor Clq, collagen alpha 1(X), and the brain-specific factor cerebellin, The expression of adipoQ is highly specific to adipose tissue in both mouse and rat, Expression of adipoQ is observed exclusively in mature fat cells as the stromal-vascular fraction of fat tissue does not contain adipoQ mRNA. In cultured 3T3-F442A and 3T3-L1 preadipocytes, hormone-induced differentiation dramatically increases the level of expression for adipoQ, Furthermore, the expression of adipoQ mRNA is significantly reduced in the adipose tissues from obese mice and humans. Whereas the biological function of this polypeptide is presently unknown, the tissue-specific expression of a putative secreted protein suggests that this factor may function as a novel signaling molecule for adipose tissue.