Removal of milk by suckling acutely increases the prolactin receptor gene expression in the lactating mouse mammary gland
Removal of milk by suckling acutely increases the prolactin receptor gene expression in the lactating mouse mammary gland
复制标题
通过哺乳去除乳汁会急剧增加哺乳期小鼠乳腺中催乳素受体基因的表达
DOI：
10.1016/s0303-7207(97)00086-5
复制
发表时间：
1997
影响因子：
4.1
通讯作者：
S. Sakai
中科院分区：
文献类型：
--
作者：
Jae Young Kim;Y. Mizoguchi;H. Yamaguchi;J. Enami;S. Sakai
为了确定哺乳对催乳素受体(PRL-R)基因表达的影响，我们测定了哺乳期小鼠乳腺中PRL-R基因的数量。哺乳第5天，仔鼠PRL-R(PRL-RL)mRNA的长型半衰期消失，前9h半衰期为12.5h，后9~15h半衰期为3.0h，给断奶24 h的小鼠喂饲后6h，PRL-RLmRNA半衰期增加2.5倍。PRL-RLmRNA在幼崽排出乳汁的乳腺中表达增加。乳汁PRL-R蛋白在断奶后减少或增加，在哺乳后减少或增加。从这些观察结果可以得出结论，在哺乳期，去除乳汁会显著增加PRL-RLmRNA的水平。
To determine the effect of suckling on the prolactin receptor (PRL-R) gene expression, we measured the quantity of PRL-R mRNA in the lactating mouse mammary gland. When the pups were separated from their mother on day 5 of lactation, the long form of PRL-R (PRL-RL) mRNA disappeared with a half-life of 12.5 h for the first 9 h and 3.0 h for the following 9–15 h. By supplying pups to mice which had been weaned for 24 h, PRL-RLmRNA increased 2.5-fold during the next 6 h-period. The increase in PRL-RLmRNA was found in the mammary glands from which the pups removed milk. The number of mammary PRL-R protein decreased or increased following weaning or following the removal of milk by suckling, respectively. From these observations, it was concluded that the removal of milk acutely increases the level of PRL-RLmRNA during lactation.