Evaluation of Portable Point-of-Care CD4 Counter with High Sensitivity for Detecting Patients Eligible for Antiretroviral Therapy
Evaluation of Portable Point-of-Care CD4 Counter with High Sensitivity for Detecting Patients Eligible for Antiretroviral Therapy
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DOI：
10.1371/journal.pone.0034319
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发表时间：
2012-04-19
期刊：
影响因子：
3.7
通讯作者：
Castelnuovo, Barbara
中科院分区：
文献类型：
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作者：
Manabe, Yukari C.;Wang, Yaping;Castelnuovo, Barbara
背景：需要准确、廉价的即时CD4+ T细胞检测技术，以便在较低水平的卫生中心或社区外展自愿咨询和检测中提供CD4+ T细胞检测结果。我们试图评估一种即时护理CD4+ T细胞计数器——Pima CD4检测系统，这是一种便携式、电池供电的台式仪器，设计用于使用适合现场使用的手指棒血液样本，并结合快速艾滋病毒检测。方法：与BD FACSCalibur（参考方法）相比，使用Pima CD4分析仪对毛细血管和静脉样本进行重复测量。采用配对学生t检验估计平均偏倚。Bland Altman图被用来评估一致性。结果：206名参与者入组，CD4计数中位数为396（范围：18-1500）。手指棒PIMA的平均偏差为-66.3个细胞/ μ L (95% CI为-83-4-49.2，P500个细胞/ μ L)，平均偏差为-120.6 (95% CI为-162.8,-78.4，P
Background: Accurate, inexpensive point-of-care CD4+ T cell testing technologies are needed that can deliver CD4+ T cell results at lower level health centers or community outreach voluntary counseling and testing. We sought to evaluate a point-of-care CD4+ T cell counter, the Pima CD4 Test System, a portable, battery-operated bench-top instrument that is designed to use finger stick blood samples suitable for field use in conjunction with rapid HIV testing.Methods: Duplicate measurements were performed on both capillary and venous samples using Pima CD4 analyzers, compared to the BD FACSCalibur (reference method). The mean bias was estimated by paired Student's t-test. Bland Altman plots were used to assess agreement.Results: 206 participants were enrolled with a median CD4 count of 396 (range; 18-1500). The finger stick PIMA had a mean bias of -66.3 cells/mu L (95% CI -83-4-49.2, P500 cells/mu L) with a mean bias of -120.6 (95% CI -162.8, -78.4, P