Spatial organization of the somatosensory cortex revealed by osmFISH
Spatial organization of the somatosensory cortex revealed by osmFISH
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DOI：
10.1038/s41592-018-0175-z
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发表时间：
2018-11-01
期刊：
影响因子：
48
通讯作者：
Linnarsson, Sten
中科院分区：
文献类型：
--
作者：
Codeluppi, Simone;Borm, Lars E.;Linnarsson, Sten
全球利用单细胞转录组学对大脑进行分子普查的努力正在产生大量分子定义的细胞类型。然而，缺乏空间信息，需要新的方法来在大的组织区域上同时绘制大量细胞类型特异性标记。在这里，我们描述了一种循环单分子荧光原位杂交方法，并定义了体感皮层的细胞组织。
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.