Structure and potential anti-fatigue mechanism of polysaccharides from Bupleurum chinense DC
Structure and potential anti-fatigue mechanism of polysaccharides from Bupleurum chinense DC
复制标题
柴胡多糖的结构及潜在抗疲劳机制
DOI：
10.1016/j.carbpol.2023.120608
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发表时间：
2023
影响因子：
11.2
通讯作者：
Lili Jiao
中科院分区：
文献类型：
--
作者：
Peng Jiang;Xiang Ji;Jing Xia;Mengran Xu;Fang Hao;Haibin Tong;Lili Jiao
从北柴胡中分离得到两种多糖：BCP-1和BCP-2。通过水提取和超滤。BCP-1（1.04 × 105 Da）和BCP-2（2.14 × 104 Da）分别由甘露糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖和半乳糖醛酸组成。它们的主链都含有寡聚半乳糖醛酸。BCP-1的主链由4-β-Galp和4，6-β-Glcp组成，并在4，6-β-Glcp的C4处分支。BCP-2的主链由3，5-α-Araf残基组成，C3处有分支。BCP-2能有效延长大鼠的强迫游泳时间，改善糖原储备和抗氧化系统，降低血尿素氮、乳酸水平，降低乳酸脱氢酶和肌酐激酶的表达。它通过调节骨骼肌中的5 '-AMP活化蛋白激酶（AMPK）和核因子红细胞2相关因子2（Nrf 2）信号通路来缓解体力疲劳。本研究表明，BCP-2比BCP-1具有更有效的抗疲劳活性，这可能与其初级和高级结构有关。
Two polysaccharides, BCP-1 and BCP-2, were obtained fromBupleurum chinenseDC. by water extraction and ultrafiltration. BCP-1 (1.04 × 105Da) and BCP-2 (2.14 × 104Da) were composed of Mannose, Rhamnose, Glucose, Galactose, Arabinose, and Galacturonic acid in different proportions. They both contained oligogalacturonides in their main chain. Besides, the backbone of BCP-1 was composed of 4-β-Galpand 4,6-β-Glcp, and branched at C4 of 4,6-β-Glcp. While BCP-2 contained a backbone of 3,5-α-Arafresidues with branches at C3. BCP-2 effectively extended the forced swimming time, improved the glycogen reserves and antioxidant system, decreased the levels of blood urea nitrogen, lactic acid, lactate dehydrogenase and creatinine kinase expression. It alleviated physical fatigue through regulating 5’-AMP-activated protein kinase (AMPK) and Nuclear Factor erythroid 2-Related Factor 2 (Nrf2) signalling pathway in skeletal muscles. This study demonstrated that BCP-2 exhibited more effective anti-fatigue activity than BCP-1 potentially associated with its primary and higher structures.