Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis
Heat induces the splicing by IRE1 of a mRNA encoding a transcription factor involved in the unfolded protein response in Arabidopsis
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拟南芥中热诱导 IRE1 编码参与未折叠蛋白反应的转录因子的 mRNA 剪接
DOI：
10.1073/pnas.1102117108
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发表时间：
2011-04-26
影响因子：
11.1
通讯作者：
Howell, Stephen H.
中科院分区：
文献类型：
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作者：
Deng, Yan;Humbert, Sabrina;Howell, Stephen H.
不利的环境条件会造成植物内质网(ER)胁迫。作为对热或内质网逆境因子的响应，拟南芥幼苗通过激活内质网相关的转录因子和RNA剪接因子IRE1b来缓解逆境伤害。IRE1b拼接编码bZIP60的mRNA，bZIP60是一个基本的亮氨酸拉链结构域，包含与植物中未折叠蛋白反应相关的转录因子。BZIP60是内质网应激反应中上调结合蛋白3(BIP3)所必需的，在BIP3诱导过程中，IRE1b功能缺失突变或bZIP60基因剪接点突变是缺陷的。这些发现表明，植物中的bZIP60是由RNA剪接激活的，并为监测和调控植物的逆境反应提供了机会。
Adverse environmental conditions produce endoplasmic reticulum (ER) stress in plants. In response to heat or ER stress agents, Arabidopsis seedlings mitigate stress damage by activating ER-associated transcription factors and a RNA splicing factor, IRE1b. IRE1b splices the mRNA-encoding bZIP60, a basic leucine-zipper domain containing transcription factor associated with the unfolded protein response in plants. bZIP60 is required for the up-regulation of BINDING PROTEIN3 (BIP3) in response to ER stress, and loss-of-function mutations in IRE1b or point mutations in the splicing site of bZIP60 mRNA are defective in BIP3 induction. These findings demonstrate that bZIP60 in plants is activated by RNA splicing and afford opportunities for monitoring and modulating stress responses in plants.