Dynamic Evolution of α-Gliadin Prolamin Gene Family in Homeologous Genomes of Hexaploid Wheat.
Dynamic Evolution of α-Gliadin Prolamin Gene Family in Homeologous Genomes of Hexaploid Wheat.
复制标题
DOI：
10.1038/s41598-018-23570-5
复制
发表时间：
2018-03-26
影响因子：
4.6
通讯作者：
Gu YQ
中科院分区：
文献类型：
--
作者：
Huo N;Zhu T;Altenbach S;Dong L;Wang Y;Mohr T;Liu Z;Dvorak J;Luo MC;Gu YQ
小麦Gli-2基因座编码α-醇溶蛋白复合组，对面包制作很重要，但也是乳糜泻(CD)的主要诱因。α-醇溶蛋白进化的阐明为培育具有更好的最终用途和降低免疫原性的小麦提供了知识。Gli-2基因座含有大量重复的基因和高度重复的DNA，使其基因组区域的序列组装具有挑战性。在这里，我们通过将基于PacBio的序列重叠群与生物纳米基因组图进行比对，构建了跨越三个小麦同源α-醇溶蛋白基因座的高质量序列。共鉴定出47个α-醇溶蛋白基因，其中只有26个编码完整的全长蛋白产物。α-醇溶蛋白基因座的分析和系统发育树的构建表明，在A、B和D基因组分化后的最后250万年内，α-醇溶蛋白基因发生了显著的复制，支持其在不同的小麦基因组中的快速扩展。我们发现，α-醇溶蛋白基因表达的显著差异不能归因于启动子区域的序列变异。这项研究还为CD表位的进化提供了洞察力，并在六倍体小麦D基因组中发现了一个可能导致剧毒33聚体Cd表位产生的单一插入事件。
Wheat Gli-2 loci encode complex groups of α-gliadin prolamins that are important for breadmaking, but also major triggers of celiac disease (CD). Elucidation of α-gliadin evolution provides knowledge to produce wheat with better end-use properties and reduced immunogenic potential. The Gli-2 loci contain a large number of tandemly duplicated genes and highly repetitive DNA, making sequence assembly of their genomic regions challenging. Here, we constructed high-quality sequences spanning the three wheat homeologous α-gliadin loci by aligning PacBio-based sequence contigs with BioNano genome maps. A total of 47 α-gliadin genes were identified with only 26 encoding intact full-length protein products. Analyses of α-gliadin loci and phylogenetic tree reconstruction indicate significant duplications of α-gliadin genes in the last ~2.5 million years after the divergence of the A, B and D genomes, supporting its rapid lineage-independent expansion in different Triticeae genomes. We showed that dramatic divergence in expression of α-gliadin genes could not be attributed to sequence variations in the promoter regions. The study also provided insights into the evolution of CD epitopes and identified a single indel event in the hexaploid wheat D genome that likely resulted in the generation of the highly toxic 33-mer CD epitope.
登录
查看更多内容
影响因子：
5.6
作者：
Leroy P;Guilhot N;Sakai H;Bernard A;Choulet F;Theil S;Reboux S;Amano N;Flutre T;Pelegrin C;Ohyanagi H;Seidel M;Giacomoni F;Reichstadt M;Alaux M;Gicquello E;Legeai F;Cerutti L;Numa H;Tanaka T;Mayer K;Itoh T;Quesneville H;Feuillet C
通讯作者：
Feuillet C
影响因子：
2
作者：
Altenbach SB;Tanaka CK;Hurkman WJ;Whitehand LC;Vensel WH;Dupont FM
通讯作者：
Dupont FM
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
9.2
作者：
Cao H;Hastie AR;Cao D;Lam ET;Sun Y;Huang H;Liu X;Lin L;Andrews W;Chan S;Huang S;Tong X;Requa M;Anantharaman T;Krogh A;Yang H;Cao H;Xu X
通讯作者：
Xu X
影响因子：
5.4
作者：
Anderson, OD;Greene, FC
通讯作者：
Greene, FC