A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs.
A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs.
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DOI：
10.4161/epi.32252
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发表时间：
2014-09
期刊：
影响因子：
3.7
通讯作者：
Osborn T
中科院分区：
文献类型：
--
作者：
Philibert RA;Penaluna B;White T;Shires S;Gunter T;Liesveld J;Erwin C;Hollenbeck N;Osborn T
酗酒对全世界数以百万计的人有深远的影响。然而，依赖自我报告阻碍了确定患者是否需要治疗的能力，临床医生监测患者对治疗的反应的能力因缺乏可靠的生物标记物而受到挑战。使用全基因组方法，我们之前已经表明，长期饮酒与人类细胞系DNA甲基化变化有关。在这项先导性研究中，我们现在检查从受试者进入和离开短期酒精治疗时收集的外周单个核细胞DNA中的DNA甲基化。与戒酒对照组相比，大量饮酒的受试者表现出广泛的DNA甲基化变化，这种变化有随着戒酒而逆转的趋势。通路分析表明，这些变化映射到参与细胞凋亡的基因网络。酒精特征与先前因吸烟而产生的甲基化特征没有显著重叠。我们得出结论，DNA甲基化可能在评估急性酒精使用状态和监测治疗反应方面具有未来的临床实用价值。
Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician’s capability to monitor the patient’s response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response.
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影响因子：
3.5
作者：
Besingi, Welisane;Johansson, Asa
通讯作者：
Johansson, Asa
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
影响因子：
5.5
作者：
Bouchery, Ellen E.;Harwood, Henrick J.;Brewer, Robert D.
通讯作者：
Brewer, Robert D.
影响因子：
2.9
作者：
Otzen, D. E.;Sehgal, P.;Nesgaard, L. W.
通讯作者：
Nesgaard, L. W.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y