A cytosol protease from the estrogen-resistant C3H mammary carcinoma increases the affinity of the estrogen receptor for DNA in vitro.
A cytosol protease from the estrogen-resistant C3H mammary carcinoma increases the affinity of the estrogen receptor for DNA in vitro.
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来自雌激素抗性 C3H 乳腺癌的胞质蛋白酶可在体外增加雌激素受体对 DNA 的亲和力。
DOI：
10.1111/j.1432-1033.1985.tb08703.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. Rochefort
中科院分区：
文献类型：
--
作者：
P. Baskevitch;H. Rochefort
我们之前的研究表明，在雌激素无反应的C3H小鼠乳腺肿瘤中，雌激素受体（ER）对小牛胸腺DNA的亲和力是子宫ER的四倍[Baskevitch， P. P., Vignon， F., Bousquet， C. and Rochefort， H. (1983) Cancer Res. 43, 2290]。通过混合来自肿瘤和子宫的细胞溶胶，我们描述了一种能够增加内质网对DNA亲和力的肿瘤因子，通过DNA-纤维素色谱和饱和度研究进行了检测。该因子的活性被α -凝乳胰蛋白酶抑制剂如n-甲苯基苯丙酰氯甲烷和凝乳抑素所抑制。利用荧光底物戊二酰甘酰基苯丙酰-n -萘酰胺，我们在C3H乳腺肿瘤细胞质中检测了这种蛋白酶。该蛋白酶和改变ER-DNA结合的因子在deae -纤维素、AcA - 44和carboline-琼脂糖层析中一起洗脱，并且对相同的抑制剂敏感。用[3H]他莫昔芬偶氮吡啶对内质网进行共价标记后，通过变性电泳可以看出，部分纯化的因子降低了雌激素受体的分子质量。我们认为内质网对DNA亲和力的增加和内质网分子大小的减小是由于同一种蛋白酶具有α -凝乳胰蛋白酶样特异性。
We have previously shown, in the estrogen-unresponsive C3H mouse mammary tumor that the affinity of the estrogen receptor (ER) for calf thymus DNA in vitro is four-times higher than that of uterine ER [Baskevitch, P. P., Vignon, F., Bousquet, C. and Rochefort, H. (1983) Cancer Res. 43, 2290]. By mixing cytosols from this tumor and uterus, we describe a tumor factor capable of increasing ER affinity for DNA, as assayed by DNA-cellulose chromatography and saturation studies. The activity of this factor was inhibited by alpha-chymotrypsin-inhibitors such as N-tosylphenylalanylchloromethane and chymostatin. Using the fluorogenic substrate glutarylglycylglycylphenylalanyl-N-naphthylamide, we assayed such a protease in the C3H mammary tumor cytosol. This protease and the factor altering ER-DNA binding were eluted together from chromatography on DEAE-cellulose, AcA 44, and carboline-agarose and were sensitive to the same inhibitors. The partially purified factor decreases the molecular mass of the estrogen receptor as seen by denaturing electrophoresis after covalent labelling of the ER with [3H]tamoxifen aziridine. We suggest that the increase of ER affinity for DNA and the decrease of ER molecular size are due to the same protease with an alpha-chymotrypsin-like specificity.
使用 [3H]他莫昔芬氮丙啶对雌激素受体进行高效且高度选择性的共价标记。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Katzenellenbogen,JA;Carlson,KE;Heiman,DF;Robertson,DW;Wei,LL;Katzenellenbogen,BS
通讯作者：
Katzenellenbogen,BS
糖皮质激素耐药性小鼠淋巴瘤 P1798 中缺乏负责生物效应的糖皮质激素受体结构域。
影响因子：
11.2
作者：
Okret,S;Stevens,YW;Carlstedt-Duke,J;Wrange,O;Gustafsson,JA;Stevens,J
通讯作者：
Stevens,J