Flow injection chemiluminescent determination of clenbuterol using GoldMag particles as carrier
Flow injection chemiluminescent determination of clenbuterol using GoldMag particles as carrier
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DOI：
10.1080/02652030600949592
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Cui, Yali
中科院分区：
文献类型：
--
作者：
Li, Zhefei;Jian, Liru;Cui, Yali
描述了一种基于GoldMag颗粒的新型流动注射化学发光（CL）酶免疫分析克仑特罗。GoldMag是一种新型的超顺磁性Fe3O4/Au复合颗粒，用作流动注入CL体系的载体。将盐酸克仑特罗与卵清蛋白（OVA）偶联物固定在GoldMag颗粒上，外加电磁场将颗粒固定在微通道中。将盐酸克仑特罗试验样品和盐酸克仑特罗多克隆抗体（Ab）注射到通道中，与GoldMag颗粒孵育。盐酸克仑特罗固定在磁性颗粒表面，与样品中的盐酸克仑特罗竞争多克隆抗体。游离的Ab或与盐酸克仑特罗结合的Ab被冲走，与Ag-Ab结合的磁性颗粒留在微通道中。加入辣根过氧化物酶（HRP）标记的山羊抗兔免疫球蛋白G (IgG)，与盐酸克仑特罗多克隆抗体反应；将多余的山羊抗兔hrp洗去。将化学发光试剂注入通道后，利用光电倍增管装置测量并记录磁颗粒表面发出的光。该方法的线性范围为0.01 ~ 0.1 ng g(-1)，克仑特罗的回收率为85 ~ 105%，RSD为3.2% （n = 11）。
A novel flow injection chemiluminescent (CL) enzyme immunoassay for clenbuterol analysis based on GoldMag particles is described. GoldMag is a new type of super-paramagnetic Fe3O4/Au composite particle used as a carrier in a flow injection CL system. Clenbuterol conjugated with ovalbumin (OVA) was immobilized onto GoldMag particles and the particles fixed in a micro-channel by an external electromagnetic field. The clenbuterol test sample and clenbuterol polyclonal antibody (Ab) were injected into the channel and incubated with GoldMag particles. Clenbuterol, immobilized on the magnetic particle surfaces, competes for polyclonal antibodies with clenbuterol in the test sample. The free Ab or Ab combined with the clenbuterol sample was washed away and the magnetic particles conjugated with Ag-Ab left in the micro-channel. Horseradish peroxidase (HRP)-labelled goat anti-rabbit immunoglobulin G (IgG) was added and reacted with clenbuterol polyclonal antibodies; excess goat anti-rabbit-HRP was then washed off. When chemiluminescent reagents were injected into the channel, emitted light from the magnetic particle surface was measured and recorded using a photomultiplier-based apparatus. The linear range of this novel method was 0.01-0.1 ng g(-1) and recovery of clenbuterol was 85-105% with a RSD of 3.2% (n = 11).