Systematic toxicity evaluation of polystyrene nanoplastics on mice and molecular mechanism investigation about their internalization into Caco-2 cells
Systematic toxicity evaluation of polystyrene nanoplastics on mice and molecular mechanism investigation about their internalization into Caco-2 cells
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聚苯乙烯纳米塑料对小鼠的系统毒性评价及其内化Caco-2细胞的分子机制研究
DOI：
10.1016/j.jhazmat.2021.126092
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发表时间：
2021-05-18
影响因子：
13.6
通讯作者：
Chen, Yabing
中科院分区：
文献类型：
--
作者：
Xu, Dihui;Ma, Yuhan;Chen, Yabing
纳米塑料(NPs)对水生和海洋生物的毒性效应日益受到人们的关注，而关于其对哺乳动物毒性评价的研究相对较少。在本研究中，我们观察了聚苯乙烯纳米粒(PS NPs)经口染毒28天后，小鼠脾、肺、肾、小肠、大肠、睾丸和脑组织中聚苯乙烯纳米粒(PS NPs)的蓄积，并鉴定了这些组织中的NPs可诱导细胞凋亡、炎症和结构紊乱。我们还发现PS纳米粒可导致血液系统损伤和脂代谢紊乱。进一步的体外研究表明，PS纳米粒可通过巨噬细胞吞噬和网状蛋白介导的内吞作用被肠上皮Caco-2细胞吸收，并导致Caco-2细胞间紧密连接的断裂。此外，我们还发现PS-NH2和PS-COOH更容易进入Caco-2细胞，这可能与PS-NH2和PS-COOH纳米粒具有较强的毒性有关。综上所述，本研究证实了NPs暴露对小鼠的毒性效应。这项研究可以对NPs在人体内的分布提供新的见解，并帮助我们评估NPs对人类的潜在生理风险。
There is a growing concern regarding the toxic effects of nanoplastics (NPs) on aquatic and marine organism, while relatively few studies about their toxicity evaluation on mammals are conducted. In the present study, we observed accumulation of polystyrene NPs (PS NPs) in mice spleen, lung, kidney, small intestine, large intestine, testis, and brain after oral exposure to PS NPs (similar to 100 nm, 10 mg/mL, 100 mu L) for 28 days, and NPs were identified to induce cell apoptosis, inflammation, and structure disorder in these tissues. We also found that PS NPs could bring about hematological system injury and lipid metabolism disorder. Further in vitro studies identified that PS NPs could be absorbed by the intestinal epithelial Caco-2 cells by macropinocytosis and clathrin-mediated endocytosis, and induced disruption of tight junction between Caco-2 cells. Moreover, we found that it was easier for PS-NH2 and PS-COOH to enter into Caco-2 cells, which may be associated with observed stronger toxicity of PS-NH2 and PS-COOH NPs. In summary, this study demonstrated that NPs exposure brings about toxic effects to mice. This study could provide new insights regarding the distribution of NPs in humans, and helps us to evaluate the potential physiological risks of NPs to human beings.