The spectrophotometric determination of cisplatin in urine, using o-phenylenediamine as derivatizing agent
The spectrophotometric determination of cisplatin in urine, using o-phenylenediamine as derivatizing agent
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DOI：
10.1081/al-100002709
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Dölen, E
中科院分区：
文献类型：
--
作者：
Anilanmert, B;Yalçin, G;Dölen, E
本文提出了一种用可见分光光度法测定尿中抗癌药顺二氨二氯铂的方法。该方法基于药物和邻苯二胺反应产物吸光度的测量。确定了最佳反应条件。产物在pH 6.2下在90 ℃下30 min内获得，在705 nm处产生最大吸光度，这远远超过顺铂本身、邻苯二胺和尿液中生物分子的吸收波长。采用标准加入法和高浓度的反应物克服了氯离子干扰和来自尿基质的其他可能干扰。该方法可在无干扰的情况下对药物进行检测和测定。反应产物在室温下在尿液中稳定5天。标准加入曲线在0.42-3.16 μ g/ml浓度范围内与顺铂的最终浓度呈线性关系。加标尿样中顺铂的检测限为8.40 μ g/ml。
A visible spectrophotometric method has been developed fur determination of the antitumour agent cis-diammindichloroplatinum (II) in urine. The method is based on the measurement of the absorbance of the reaction product of the drug and o-phenylenediamine. Optimum conditions of the reaction were established. The product was obtained at pH 6.2; in 30 min at 90 degreesC, giving a maximum absorbance at 705 nm, which is far beyond the wavelengths of the absorption of cisplatin itself, o-phenylenediamine, and the biomolecules in urine. The chloride ion interference and the other possible interferences originated from the urine matrix were overcome by using the standard addition method and also the high concentration of the reactant. The drug could be detected and determined without any interference. The reaction product was stable in urine for 5 days at ambient temperature. The standard addition curve was linear in the concentration range of 0.42-3.16 mug/ml in respect to the final concentration of cisplatin. The detection limit of cisplatin in spiked urine samples was 8.40 mug/ml.