Molecular and cell biology aspects of plague
Molecular and cell biology aspects of plague
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DOI：
10.1073/pnas.97.16.8778
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发表时间：
2000-08-01
影响因子：
11.1
通讯作者：
Cornelis, GR
中科院分区：
文献类型：
--
作者：
Cornelis, GR
一个70 kb的毒力质粒（有时称为pYV）使耶尔森氏菌。在宿主的淋巴组织中生存和繁殖。它编码Yop蛋白，一种由称为Yops的分泌蛋白及其专用的称为Ysc的III型分泌装置组成的系统。Ysc装置形成由29种蛋白组成的通道。其中10个在几乎每一个第三类系统中都有对应机构。一些Yops的分泌需要细菌胞质溶胶中称为Syc蛋白的小分子伴侣的帮助。这些分子伴侣充当其伴侣Yop的保镖或分泌领航员，Yop蛋白分为两类。一些是细胞内效应物，而另一些是将效应物穿过真核细胞质膜递送到真核细胞中所需的“转运子”。易位子（YopB、YopD、LcrV）在真核细胞质膜中形成16-23埃的孔。效应器Yop是YopE、YopH、YpkA/YopO、YopP/ YopJ、YopM和YopT。YopH是一种强大的磷酸酪氨酸磷酸酶，通过使几种粘着斑蛋白去磷酸化而发挥抗吞噬作用。YopE和YopT通过使控制细胞骨架动力学的GTP酶失活而有助于抗吞噬作用。YopP/YopJ通过阻止转录因子NF-κ B的活化而发挥抗炎作用。它还诱导巨噬细胞的快速凋亡。关于磷酸丝氨酸激酶YopO/YpkA和YopM的作用知之甚少。
A 70-kb virulence plasmid (sometimes called pYV) enables Yersinia spp. to survive and multiply in the lymphoid tissues of their host. It encodes the Yop virulon, a system consisting of secreted proteins called Yops and their dedicated type III secretion apparatus called Ysc, The Ysc apparatus forms a channel composed of 29 proteins. Of these, 10 have counterparts in almost every type III system. Secretion of some Yops requires the assistance, in the bacterial cytosol, of small individual chaperones called the Syc proteins. These chaperones act as bodyguards or secretion pilots for their partner Yop, Yop proteins fall into two categories. Some are intracellular effecters, whereas the others are "translocators" needed to deliver the effecters across the eukaryotic plasma membrane, into eukaryotic cells. The translocators (YopB, YopD, LcrV) form a pore of 16-23 Angstrom in the eukaryotic cell plasma membrane. The effector Yops are YopE, YopH, YpkA/YopO, YopP/ YopJ, YopM, and YopT. YopH is a powerful phosphotyrosine phosphatase playing an antiphagocytic role by dephosphorylating several focal adhesion proteins. YopE and YopT contribute to antiphagocytic effects by inactivating GTPases controlling cytoskeleton dynamics. YopP/YopJ plays an anti-inflammatory role by preventing the activation of the transcription factor NF-KB. It also induces rapid apoptosis of macrophages, Less is known about the role of the phosphoserine kinase YopO/YpkA and YopM.