Protein expression of CD44 (standard and variant isoforms) in hepatocellular carcinoma: relationships with tumor grade, clinicopathologic parameters, p53 expression, and patient survival
Protein expression of CD44 (standard and variant isoforms) in hepatocellular carcinoma: relationships with tumor grade, clinicopathologic parameters, p53 expression, and patient survival
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DOI：
10.1016/s0168-8278(00)80192-0
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发表时间：
2000-01-01
影响因子：
25.7
通讯作者：
Terada, T
中科院分区：
文献类型：
--
作者：
Endo, K;Terada, T
背景/目标。CD44家族的成员是主要作为透明质酸受体的跨膜糖蛋白。方法：采用免疫组织化学方法检测107例经福尔马林固定、石蜡包埋的肝细胞癌组织标本中不同形态CD44的表达。结果：CD44s阳性36例(34%)，CD44v552例(49%)，CD44v629例(27%)，CD44v7-841例(38%)，CD44v1026例(24%)。CD44v6在分化较差的肝癌中的高表达与血管侵犯和P53的过度表达显著相关，Kaplan-Meier对患者生存的检验表明，这五种分子中每一种都阳性的肾癌患者的生存率降低，并且所有五种CD44分子均阳性的肝细胞癌患者的生存率比FDR阳性的肝细胞癌患者差。在多因素生存分析中，CD44s阳性是一个独立因素。然而，一个或多个CD44亚型的阳性表达是影响总生存率的最有用的独立因素。结论：CD44亚型的上调与低分化肝细胞癌和生存期缩短有关。
Background/Aims. Members of the CD44 family are transmembrane glycoproteins which act mainly as receptors for hyaluronan. We have examined the expression of CD44s and several CD44v and the relationship between these and hepatocellular carcinoma (HCC) grade, clinicopathological parameters, p53 expression, and patient survival in T-TCC,Methods: Formalin-fixed, paraffin-embedded tissue sections from 107 surgically resected HCC were examined immunohistochemically using a semi-quantitative scoring system to detect the expression of different forms of CD44.Results: The number of CD44s-positive cases was 36 (34%), CD44v5 52 (49%), CD44v6 29 (27%), CD44v7-8 41 (38%), and CD44v10 26 (24%), Expression of these molecules correlated with high histological grade, being the highest in poorly-daferentiated HCC, High CD44v6 expression significantly correlated with the presence of vascular invasion and p53 overexpression, Kaplan-Meier examination of patient survival revealed that RCC patients with positivity of each of these five molecules had a reduced survival rate, and that HCC patients positive for all the five CD44 molecules had worse survival than HCC patients positive fdr four or less of these CD44 molecules. in multivariate survival analysis, CD44s positivity was an independent factor. However, positivity for one or more CD44 isoforms was the most useful independent factor for overall survival.Conclusion: These results suggest that up-regulation of CD44 isoforms is associated with poorly-differentiated HCC and shortened survival.