An actuated pump on-chip powered by cultured cardiomyocytes
An actuated pump on-chip powered by cultured cardiomyocytes
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DOI：
10.1039/b515149j
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发表时间：
2006-03-01
期刊：
影响因子：
6.1
通讯作者：
Kitamori, T
中科院分区：
文献类型：
--
作者：
Tanaka, Y;Morishima, K;Kitamori, T
细胞功能经常被用作综合化学系统（如生化反应器和生物测定系统）的处理组件。在这里，我们创造了一个新的基于细胞的微系统，利用心肌细胞固有的脉冲机械功能，构建了一个细胞微泵芯片，使用心肌细胞片作为原型生物微致动器。我们首先在一个没有止回阀的模型微通道中演示了基于细胞的流体运动控制，并评估了生物驱动的潜在性能。为此，将聚二甲基硅氧烷（PDMS）微芯片与配备有隔膜和能够利用集体细胞流体机械力的推杆结构的微通道耦合到培养的脉动心肌细胞片上，通过驱动隔膜来激活微通道中基于细胞的流体运动。该系统中细胞振荡频率和相关流体位移随温度的变化而变化。随着培养温度的升高，集体细胞的收缩频率保持协同同步，但有所增加，位移略有减少。然后，我们演示了使用聚酰亚胺制成的悬臂式微止回阀在微通道内定向泵送流体。产生的定向流速为nL min（-1）。这种细胞微泵系统可以进一步发展为一种自驱动的、高效的机械化学换能器，在未来的各种用途中不需要外部能源。
Cellular functions are frequently exploited as processing components for integrated chemical systems such as biochemical reactors and bioassay systems. Here, we have created a new cell-based microsystem exploiting the intrinsic pulsatile mechanical functions of cardiomyocytes to build a cellular micropump on-chip using cardiomyocyte sheets as prototype bio-microactuators. We first demonstrate cell-based control of fluid motion in a model microchannel without check valves and evaluate the potential performance of the bio-actuation. For this purpose, a poly( dimethylsiloxane) ( PDMS) microchip with a microchannel equipped with a diaphragm and a push-bar structure capable of harnessing collective cell fluid mechanical forces was coupled to a cultured pulsating cardiomyocyte sheet, activating cell-based fluid movement in the microchannel by actuating the diaphragm. Cell oscillation frequency and correlated fluid displacement in this system depended on temperature. When culture temperature was increased, collective cell contraction frequency remained cooperative and synchronous but increased, while displacement was slightly reduced. We then demonstrated directional fluid pumping within microchannels using cantilever-type micro-check valves made of polyimide. A directional flow rate of nL min(-1) was produced. This cell micropump system could be further developed as a self-actuated and efficient mechanochemical transducer requiring no external energy sources for various purposes in the future.