A ROLE FOR FIBRONECTIN IN THE MIGRATION OF AVIAN PRECARDIAC CELLS .1. DOSE-DEPENDENT EFFECTS OF FIBRONECTIN ANTIBODY
A ROLE FOR FIBRONECTIN IN THE MIGRATION OF AVIAN PRECARDIAC CELLS .1. DOSE-DEPENDENT EFFECTS OF FIBRONECTIN ANTIBODY
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DOI：
10.1016/0012-1606(88)90378-8
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发表时间：
1988-10-01
影响因子：
2.7
通讯作者：
LASH, JW
中科院分区：
文献类型：
--
作者：
LINASK, KK;LASH, JW
与早期鸡胚内胚层相关的纤维连接蛋白的前后浓度差异与心前中胚层细胞的定向迁移有关。我们已经研究了增加浓度的纤维连接蛋白（FN）抗体的效果，以测试FN对心前细胞迁移的重要性。对于对照，将胚胎在针对几种其他细胞外组分（如层粘连蛋白和抗胶原I型和IV型）以及针对整联蛋白（细胞表面FN受体）产生的抗体的存在下孵育。胚胎还在高浓度的外源性FN的存在下孵育，以及在FN受体识别的含RGD的合成五肽的存在下孵育。在不同浓度的抗-FN（5至80 μ g/ml）中孵育鸡胚后，观察到抗-纤连蛋白的剂量依赖性作用，由此心脏发育在高浓度的抗-FN下被阻止。早期发育阶段更容易受到较低的抗体浓度比后期。在含有RGD的合成肽的存在下孵育导致部分心裂。作为对照的抗体都不影响细胞迁移或早期心脏发育。这些结果支持FN是迁移途径中的主要成分的假设，并在心前细胞向胚胎中线的定向迁移中发挥作用。
An anterior-posterior concentration difference of fibronectin associated with the endoderm in early chick embryos has been implicated in the directional migration of precardiac mesoderm cells. We have examined the effect of increasing concentrations of an antibody to fibronectin (FN) to test the essentiality of FN to precardiac cell migration. For controls embryos were incubated in the presence of antibodies produced against several other extracellular components, such as laminin and anti-collagen types I and IV, as well as against integrin, a cell surface FN receptor. Embryos were also incubated in the presence of a high concentration of exogenous FN, as well as in the presence of an RGD-containing synthetic pentapeptide that is recognized by the FN receptor. After incubation of chick embryos in various concentrations of anti-FN(5 to 80 .mu.g/ml), a dose-dependent effect of anti-fibronectin was observed, whereby heart development was arrested at high concentrations of anti-FN. Early developmental stages were more susceptible to lower antibody concentrations than later stages. Incubation in the presence of the RGD-containing synthetic peptide resulted in partial cardiabifida. None of the antibodies serving as controls affected cell migration or early heart development. These results support the hypothesis that FN is a major component in the migratory pathway and plays a role in the directional migration of precardiac cells to the embryonic midline.