Possible involvement of eEF1A in Tomato spotted wilt virus RNA synthesis
Possible involvement of eEF1A in Tomato spotted wilt virus RNA synthesis
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DOI：
10.1016/j.virol.2014.07.053
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发表时间：
2014-11-01
期刊：
影响因子：
3.7
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Komoda, Keisuke;Ishibashi, Kazuhiro;Ishikawa, Masayuki
番茄斑萎病毒（Tomato spotted wilt virus，TSWV）是布尼亚病毒科的一种负链RNA病毒，在昆虫和植物中均可传播。虽然TSWV可以感染广泛的植物物种，但植物中TSWV的病毒RNA合成所涉及的宿主因素尚未被表征。在这份报告中，我们证明，来自宿主植物之一的无细胞提取物可以激活TSWV的mRNA转录活性。基于无细胞提取物的活性导向分馏，我们确定了真核延伸因子（eEF）1A作为一个可能的宿主因子，促进TSWV的转录和复制。在eEF1A抑制剂存在下，RNA合成支持活性降低，表明eEF1A在TSWV的RNA合成中起重要作用。(C)2014爱思唯尔公司All rights reserved.
Tomato spotted wilt virus (TSWV) is a negative-strand RNA virus in the family Bunyaviridae and propagates in both insects and plants. Although TSWV can infect a wide range of plant species, host factors involved in viral RNA synthesis of TSWV in plants have not been characterized. In this report, we demonstrate that the cell-free extract derived from one of the host plants can activate mRNA transcriptional activity of TSWV. Based on activity-guided fractionation of the cell-free extract, we identified eukaryotic elongation factor (eEF) 1A as a possible host factor facilitating TSWV transcription and replication. The RNA synthesis-supporting activity decreased in the presence of an eEF1A inhibitor, suggesting that eEF1A plays an important role in RNA synthesis of TSWV. (C) 2014 Elsevier Inc. All rights reserved.