Protective effect of salidroside against H2O2-induced cell apoptosis in primary culture of rat hippocampal neurons
Protective effect of salidroside against H2O2-induced cell apoptosis in primary culture of rat hippocampal neurons
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DOI：
10.1007/s11010-009-0177-3
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发表时间：
2009-12-01
影响因子：
4.3
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Chen, Xia;Zhang, Qi;Ding, Fei
红景天苷是从药用植物红景天中分离得到的一种苯丙素苷类化合物，已被证明对体外培养的神经细胞具有保护作用。本研究探讨红景天苷是否能够延长其独特的神经保护作用，以原代培养的大鼠海马神经元对过氧化氢（H2 O2）诱导的细胞损伤。细胞活力测试和细胞凋亡测定证实，红景天苷预处理衰减H2 O2刺激的海马神经元的原代培养中的细胞凋亡以浓度依赖性的方式。对caspase-3活性、一氧化氮（NO）生成和一氧化氮合酶（NOS）活性的测定表明，红景天苷的保护作用可能是通过抑制caspase-3活性、拮抗H2 O2刺激时NO生成和NOS活性来实现的。也许，这项研究可能有助于开发红景天苷作为一种广谱药物，用于预防和/或治疗神经退行性疾病中的神经元损伤。
Salidroside, a phenylpropanoid glycoside separated from a medicinal plant Rhodiola rosea, has been documented to have protective effects on neuronal cells in vitro. This study investigated whether salidroside was able to extend its unique neuroprotection to primary cultured rat hippocampal neurons against hydrogen peroxide (H2O2)-induced cell damage. Cell viability tests and cell apoptosis assays confirmed that salidroside pretreatment attenuated H2O2-stimulated apoptotic cell death in primary culture of hippocampal neurons in a concentration-dependent manner. The measurements of caspase-3 activity, nitric oxide (NO) production, and NO synthase (NOS) activity suggest that the protection of salidroside, shown in this study, might be mediated by inhibiting caspase-3 activity, and antagonizing NO production and NOS activity during H2O2 stimulation. Perhaps, this study might contribute to the development of salidroside as a broad-spectrum agent for preventing and/or treating neuronal damage in neurodegenerative disorders.