β1-integrins determine the dendritic morphology which enhances DC-SIGN-mediated particle capture by dendritic cells
β1-integrins determine the dendritic morphology which enhances DC-SIGN-mediated particle capture by dendritic cells
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DOI：
10.1093/intimm/dxl062
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发表时间：
2006-08-01
影响因子：
4.4
通讯作者：
Chain, Benjamin M.
中科院分区：
文献类型：
--
作者：
Andersen, Claire A. Swetman;Handley, Matthew;Chain, Benjamin M.
抗原呈递树突状细胞（DC）的形态特征是存在大量长树突。这些过程的形成需要 DC 表面的 β1-整合素 (CD29) 和细胞外基质中的纤连蛋白之间的相互作用。这种相互作用发生在树突尖端形成的焦点接触处，树突尖端含有高浓度的 β1-整合素、肌动蛋白和细胞骨架蛋白纽蛋白、桩蛋白和踝蛋白。树突含有广泛的微管（MT）网络，并且在 MT 抑制剂秋水仙碱存在下会收缩，这表明 MT 对于树突稳定性至关重要。研究表明，树突状形态可直接增强捕获树突状细胞特异性 ICAM-3 抓取非整联蛋白 (DC-SIGN) 包被珠的能力。延时摄影表明，树突是高度动态的结构，细胞在 3 小时内向不同方向延伸和缩回多个树突。这种运动能力使单个 DC 扫描的面积增加了 2 倍以上。树突形态和高运动性的不寻常组合可能在 DC 作为免疫系统哨兵的有效功能中发挥重要作用。
The morphology of antigen-presenting dendritic cells (DCs) is characterized by the presence of numerous long dendrites. The formation of these processes is shown to require the interaction between the beta 1-integrin (CD29) on the surface of the DCs and fibronectin in the extracellular matrix. This interaction occurs at focal contacts formed at the tips of dendrites, which contain high concentrations of the beta 1-integrins, actin and the cytoskeletal proteins vinculin, paxillin and talin. Dendrites contain an extensive microtubule (MT) network, and are retracted in the presence of the MT inhibitor colchicine, suggesting that MTs are essential for dendrite stability. The dendritic morphology is shown to contribute directly to an enhanced ability to capture dendritic cell specific ICAM-3 grabbing nonintegrin (DC-SIGN)-coated beads. Time-lapse photography demonstrates that dendrites are highly dynamic structures, with cells extending and retracting multiple dendrites in different directions over a 3-h period. This motility increases the area scanned by an individual DC by over 2-fold. The unusual combination of a dendritic morphology and high motility is likely to play a major role in the efficient function of DCs as sentinels of the immune system.