An ultrasensitive electrochemical immunosensor for apolipoprotein E4 based on fractal nanostructures and enzyme amplification
An ultrasensitive electrochemical immunosensor for apolipoprotein E4 based on fractal nanostructures and enzyme amplification
复制标题
基于分形纳米结构和酶放大的载脂蛋白E4超灵敏电化学免疫传感器
DOI：
10.1016/j.bios.2015.04.068
复制
发表时间：
2015-09-15
影响因子：
12.6
通讯作者：
Zhang, Xueji
中科院分区：
文献类型：
--
作者：
Liu, Yibiao;Xu, Li-Ping;Zhang, Xueji
人类载脂蛋白 E4 (APOE4) 是阿尔茨海默病 (AD) 的主要危险因素，可大大增加发病率。本工作基于分形金（FracAu）纳米结构和酶放大设计了一种用于定量检测 APOE4 的超灵敏夹心型电化学免疫传感器。 FracAu 纳米结构通过四氯金酸氢 (HAuCl4) 直接电沉积在聚电解质修饰的氧化铟锡 (ITO) 电极上。通过循环伏安法（CV）研究了修饰界面的传感性能。用 HRP 标记的 APOE4 抗体功能化后，可以通过电流响应定量检测人 APOE4。电流响应与人 APOE4 浓度的对数在 1.0 至 10,000.0 ng/mL 范围内呈线性关系，检测限为 0.3 ng/mL。制备的APOE4电化学免疫传感器具有特异性强、灵敏度高、检测限低、线性范围宽等特点。人类APOE4的检测为AD的预防和易感人群的早期预警提供了有力的支持。 (C) 2015 Elsevier B.V. 保留所有权利。
Human apolipoprotein E4 (APOE4) is a major risk factor for Alzheimer's disease (AD) and can greatly increase the morbidity. in this work, an ultrasensitive sandwich-type electrochemical immunosensor for the quantitative detection of APOE4 was designed based on fractal gold (FracAu) nanostructures and enzyme amplification. The FracAu nanostructures were directly electrodeposited by hydrogen tetrachloroaurate (HAuCl4) on polyelectrolytes modified indium tin oxide (ITO) electrode. The sensing performances of the modified interface were investigated by cyclic voltammetry (CV). After functionalization with HRP-labeled APOE4 antibody, the human APOE4 could be detected quantitatively by current response. The current response has a linear relationship with the logarithm of human APOE4 concentrations in a range from 1.0 to 10,000.0 ng/mL, with a detection limit of 0.3 ng/mL. The fabricated APOE4 electrochemical immunosensor exhibits strong specificity, high sensitivity, low detection limit and wide linear range. The detection of human APOE4 provides a strong support for the prevention of AD and early-stage warning for those susceptible populations. (C) 2015 Elsevier B.V. All rights reserved.