Behavioral Changes and Hippocampus Glucose Metabolism in APP/PS1 Transgenic Mice via Electro-acupuncture at Governor Vessel Acupoints.
Behavioral Changes and Hippocampus Glucose Metabolism in APP/PS1 Transgenic Mice via Electro-acupuncture at Governor Vessel Acupoints.
复制标题
电针督脉腧穴对APP/PS1转基因小鼠行为变化及海马葡萄糖代谢的影响
DOI：
10.3389/fnagi.2017.00005
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发表时间：
2017
影响因子：
4.8
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Cao J;Tang Y;Li Y;Gao K;Shi X;Li Z
目的：采用Morris水迷宫（MWM）空间学习记忆行为测试结合micro-PET（micro-positron emission tomography，micro-PET）海马葡萄糖代谢显像，观察电针治疗对阿尔茨海默病（Alzheimer's disease，AD）小鼠的影响。研究方法：将30只7月龄APP/PS1小鼠随机分为AD模型组（AD组）、药物组（M组）和电针组，C57 BL/6小鼠为正常对照组（N组），每组10只。M组给予多奈哌齐0.92mg/kg灌胃干预。电针百会穴、印堂穴20 min后刺水沟穴，N、M、AD组给予束缚20 min，每天1次，连续15 d。给药后采用MWM观察小鼠行为学变化，采用micro-PET成像检测小鼠海马葡萄糖代谢水平。结果如下：与AD组相比，M组和EA组大鼠逃避潜伏期明显缩短（P < 0.01），平台象限游泳距离占总游泳距离的比例明显增加（P < 0.01），EA组所占比例高于M组。micro-PET显像显示AD组小鼠海马葡萄糖代谢率低于N组（P < 0.01）。M组和EA组注射剂量均显著高于AD组（P < 0.01），EA组摄取率高于M组。结论：多奈哌齐和电针对AD小鼠均有治疗作用。在一定程度上，电针通过改善AD大鼠的空间学习记忆能力，同时增强海马葡萄糖代谢，显示出较好的治疗AD的疗效。
Objective: Investigating the effects of electro-acupuncture (EA) treatment on mice with Alzheimer’s disease (AD), using Morris water maze (MWM) for spatial learning and memory behavior tests combined with micro-positron emission tomography (micro-PET) imaging for glucose metabolism in hippocampus. Methods: Thirty seven-month-old APP/PS1 mice were randomly divided into AD Model group (AD group), medicine group (M group) and EA group, C57BL/6 mice were used for Normal control group (N group), n = 10 in each group. Mice in M group received donepezil intervention by gavage with dose at 0.92 mg/kg. EA was applied at Baihui (GV20) and Yintang (GV29) acupoints for 20 min then pricked at Shuigou (GV26) acupoint, while mice in N, M and AD groups were received restriction for 20 min, with all treatment administrated once a day for 15 consecutive days. After the treatment, MWM was performed to observe behavioral changes in mice, then hippocampus glucose metabolism level was tested by micro-PET imaging. Results: Compared with that of AD group, the escape latency of M and EA groups declined significantly (P < 0.01), while the proportion of the platform quadrant swimming distance in total swimming distance showed an obvious increase (P < 0.01), and EA group occupied a higher percentage than that in M group. The micro-PET imaging showed that mice in AD group performed a lower glucose metabolic rate in hippocampus compared with N group (P < 0.01). Both M and EA groups presented a significant higher injected dose compared with AD group (P < 0.01), and the uptake rate of EA group was higher than M group. Conclusion: Both donepezil and EA have therapeutic effects on AD mice. To a certain extent, EA shows a better efficacy in treatment of AD by improving the spatial learning and memory ability, while also enhancing glucose metabolism in hippocampus.