Berberine synergy with amphotericin B against disseminated candidiasis in mice
Berberine synergy with amphotericin B against disseminated candidiasis in mice
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DOI：
10.1248/bpb.28.541
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发表时间：
2005-03-01
影响因子：
2
通讯作者：
Lee, JH
中科院分区：
文献类型：
--
作者：
Han, Y;Lee, JH
在本研究中，我们研究了黄连素对由致病真菌白色念珠菌引起的播散性念珠菌病的协同作用。黄连素在体外条件下对白色念珠菌的生长有抑制作用。肉汤敏感性试验显示黄连素与两性霉素B(Amp B)有协同作用。为了在体内条件下证实这些结果，在小鼠身上检测了其对播散性念珠菌病的影响。结果显示，给予稀释剂(阴性对照)、Amp B(0.5 mg/kg体重)或黄连素(1 mg/kg体重)的小鼠的平均存活时间(MST)分别约为12、14和17天。相反，在相同浓度下联合使用这两种药物的小鼠的MST值为36d，比仅使用Amp B的小鼠平均多存活22d。该MST值与给予4倍Amp B剂量的小鼠的MST值几乎相同。这些数据表明，Amp B和黄连素的组合可以减少约75%的Amp B剂量，这意味着黄连素确实与Amp B具有协同作用来对抗播散性疾病。
In present study, we investigated the synergic effect of berberine against disseminated candidiasis caused by the pathogenic fungus, Candida albicans. Berberine inhibited the growth of C albicans under in-vitro condition. The broth susceptibility revealed the synergic effect of berberine with amphotericin B (Amp B). To confirm these results under the in-vivo condition, the effect was examined in mice against disseminated candidiasis. Results showed mice that were given diluent (negative control), Amp B (0.5 mg/kg of body weight), or berberine (1 mg/kg of body weight) had mean survival times (MST) of approximately 12, 14, and 17 d, respectively. On the contrary, mice that were treated using a combination of the two agents at the same concentrations resulted in a MST value of 36 d, surviving at an average of 22 d longer than the mice group treated only with the Amp B. This MST value was almost same as MST value from the mice that were given four times the Amp B dose. These data indicate that the combination of Amp B and berberine could reduce approximately 75% of the Amp B dose, implying that berberine indeed has synergy with Amp B against the disseminated disease.