Distinct Dynamics of Stem and Progenitor Cells in Blood of Polytraumatized Patients.
Distinct Dynamics of Stem and Progenitor Cells in Blood of Polytraumatized Patients.
复制标题
多发性创伤患者血液中干细胞和祖细胞的独特动态。
DOI：
10.1097/shk.0000000000001198
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Geiger,Hartmut
中科院分区：
文献类型：
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作者：
Vogel,Mona;Christow,Hannes;Manz,Isabel;Denkinger,Michael;Amoah,Amanda;Schütz,Desiree;Brown,Andreas;Möhrle,Bettina;Schaffer,Annika;Kalbitz,Miriam;Gebhard,Florian;Mayer,Benjamin;Huber-Lang,Markus;Geiger,Hartmut
内源性动员的干细胞和祖细胞（SPC）或外源性提供的SPC被认为是有益的创伤治疗。然而，对于严重损伤后血液中SPC数量的同步动态以及与这些数量相关的细胞因子谱等参数仍然知之甚少。我们测定了多发性创伤患者（损伤严重程度评分≥ 21）外周血（PB）中造血干细胞、普通髓系祖细胞、粒细胞-巨噬细胞祖细胞和间充质干细胞/基质细胞的数量（0至3、8、24、48和120 h）。我们发现，血液SPC的数量平均遵循同步的反钟形分布，在0至3小时增加，随后强烈下降，血液中SPC数量在24或48小时达到最低点。在48 h和120 h之间PB中SPC数量的变化揭示了两种不同的模式：模式1的特征在于SPC数量增加到高于正常的水平，模式2的特征在于与最低点相比SPC数量几乎没有增加。细胞因子CK、MDC、IL-8、G-CSF Gro-α、VEGF和MCP-1浓度的变化与PB中SPC数量的变化相关，或与模式1或模式2密切相关。我们的数据为研究多发性创伤患者干细胞动员的作用及其对创伤结局可能的积极影响提供了新的理论基础。
Endogenously mobilized stem and progenitor cells (SPCs) or exogenously provided SPCs are thought to be beneficial for trauma therapy. However, still little is known about the synchronized dynamics of the number of SPCs in blood after severe injury and parameters like cytokine profiles that correlate with these numbers. We determined the number of hematopoietic stem cells, common myeloid progenitors, granulocyte-macrophage progenitors, and mesenchymal stem/stromal cells in peripheral blood (PB) 0 to 3, 8, 24, 48, and 120 h after polytrauma in individual patients (injury severity score≥ 21). We found that the number of blood SPCs follows on average a synchronous, inverse bell-shaped distribution, with an increase at 0 to 3 h, followed by a strong decrease, with a nadir in SPC numbers in blood at 24 or 48 h. The change in numbers of SPCs in PB between 48 h and 120 h revealed two distinct patterns: Pattern 1 is characterized by an increase in the number of SPCs to a level higher than normal, pattern 2 is characterized by an almost absent increase in the number of SPCs compared to the nadir. Changes in the concentrations of the cytokines CK, MDC, IL-8, G-CSF Gro-α, VEGF, and MCP-1 correlated with changes in the number of SPCs in PB or were closely associated with Pattern 1 or Pattern 2. Our data provide novel rationale for investigations on the role of stem cell mobilization in polytraumatized patients and its likely positive impact on trauma outcome.