Adaptation of plum pox virus to a herbaceous host (Pisum sativum) following serial passages.
Adaptation of plum pox virus to a herbaceous host (Pisum sativum) following serial passages.
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李痘病毒连续传代后对草本宿主（Pisum sativum）的适应。
DOI：
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发表时间：
2007
影响因子：
3.8
通讯作者：
W. Schneider
中科院分区：
文献类型：
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作者：
C. Wallis;A. Stone;D. Sherman;V. Damsteegt;F. Gildow;W. Schneider
桃树上的李痘病毒(PPV)种群能够一致地适应草本寄主，其特征是症状形成时间缩短，接种效率提高，滴度增加。以豌豆为替代寄主，研究了豌豆对PPV的适应性。将桃PPV的两个分离物接种豌豆，分别用蚜虫和机械接种方法传代10次，获得4个独立的传代系。在豌豆上，从桃子到豌豆的机械传动效率从第1代的3%提高到第4代的100%。利用蚜虫媒介接种豌豆需要6-10代传代才能达到50-60%的传播效率峰值。对先后接种豌豆的4个PPV群体株系的序列分析表明，与在桃树上平行传代的相同PPV分离物相比，在NIB基因上发生了一致的特异性突变。这种突变使PPV在新宿主中的复制速度提高了20倍。每一代豌豆适应的PPV菌株也被测试了它们感染原始宿主桃子的能力。无论以前的传代次数多少，所有豌豆适应的PPV菌株都在接种蚜虫的情况下持续低水平侵染桃树。
Plum pox virus (PPV) populations from peaches are able to adapt consistently to herbaceous hosts, characterized by a reduction in time to symptom development, increases in inoculation efficiency and increased titres. PPV adaptation was studied by using pea (Pisum sativum) as an alternative host. Two isolates of PPV from peaches were inoculated and passaged in peas ten times using either aphid or mechanical inoculation, generating four independent passage lines. Mechanical-transmission efficiency from peach to pea improved from 3 % at passage 1 to 100 % by serial passage 4 on peas. Inoculation using aphid vectors required six to ten serial passages in pea to reach a peak of 50-60 % transmission efficiency. Sequence analyses of all four PPV population lines inoculated sequentially to pea identified a specific mutation occurring consistently in the NIb gene when compared with the same PPV isolates passaged in parallel in peach. The mutation allowed PPV to replicate up to 20 times faster in the new host. Pea-adapted strains of PPV at every passage were also tested for their ability to infect the original host, peach. Regardless of the number of previous passages, all pea-adapted PPV strains consistently infected peach at low levels using aphid inoculation.
影响因子：
7.2
作者：
Carrington, JC;Jensen, PE;Schaad, MC
通讯作者：
Schaad, MC