Discovery and Prevalidation of Salivary Extracellular microRNA Biomarkers Panel for the Noninvasive Detection of Benign and Malignant Parotid Gland Tumors
Discovery and Prevalidation of Salivary Extracellular microRNA Biomarkers Panel for the Noninvasive Detection of Benign and Malignant Parotid Gland Tumors
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DOI：
10.1158/1078-0432.ccr-12-3505
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发表时间：
2013-06-01
影响因子：
11.5
通讯作者：
Wong, David T. W.
中科院分区：
文献类型：
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作者：
Matse, Johannes H.;Yoshizawa, Janice;Wong, David T. W.
目的：本研究旨在探讨腮腺恶性肿瘤和良性肿瘤患者唾液微RNA（microRNA，miRNA）表达谱的差异，为腮腺恶性肿瘤和良性肿瘤的术前诊断提供依据。实验设计：腮腺恶性肿瘤患者（n = 38）和腮腺良性肿瘤患者（n = 29）的全唾液样本均来自唾液腺肿瘤生物库（SGTB）。总RNA分离后，使用人miRNA卡进行miRNA谱分析。采用双侧Wilcoxon检验分析差异miRNA表达。使用定量实时PCR（qRT-PCR）来验证独立样品组中所选的miRNA。结果：在750个检测的miRNA中，有57个存在差异表达，其中54个在恶性肿瘤中的表达高于良性肿瘤。验证9个miRNA的独立样本组中的表达揭示了与良性样本相比，恶性样本中miRNA的表达确实更高。6种验证的miRNAs的表达在两组间差异有统计学意义（P
Purpose: This study was conducted to explore the differences in salivary microRNA (miRNA) profiles between patients with malignant or benign parotid gland tumors as a potential preoperative diagnostic tool of tumors in the salivary glands.Experimental Design: Whole saliva samples from patients with malignant (n = 38) or benign (n = 29) parotid gland tumors were obtained from the Salivary Gland Tumor Biorepository (SGTB). After total RNA isolation, human miRNA cards were used for miRNA profiling. The differential miRNA expression was analyzed using two- sided Wilcoxon test. Quantitative real-timePCR (qRT-PCR) was used to validate selected miRNAs in an independent sample set. Receiver-operating characteristics curve and probability of malignancy was exploited to evaluate the diagnostic power of the validated miRNAs.Results: With miRNA profiling, 57 of 750 investigated miRNAs were differently expressed, of which 54 showed higher miRNA expression in samples from patients with malignant tumors than those from patients with benign tumors. Validating the expression in an independent sample set of 9 miRNAs revealed indeed higher expression of miRNAs in malignant samples compared with benign samples. The expression of 6 validated miRNAs was statistically significantly different between the two groups (P