ESTABLISHMENT AND CHARACTERIZATION OF 2 CELL-LINES WITH DIFFERENT GRADE OF DIFFERENTIATION DERIVED FROM ONE PRIMARY HUMAN PANCREATIC ADENOCARCINOMA
ESTABLISHMENT AND CHARACTERIZATION OF 2 CELL-LINES WITH DIFFERENT GRADE OF DIFFERENTIATION DERIVED FROM ONE PRIMARY HUMAN PANCREATIC ADENOCARCINOMA
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DOI：
10.1007/bf02890431
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发表时间：
1992-02-01
期刊：
影响因子：
--
通讯作者：
KERN, HF
中科院分区：
文献类型：
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作者：
ELSASSER, HP;LEHR, U;KERN, HF
从人类胰腺癌的肝转移中，我们建立了用于研究该肿瘤细胞生物学的细胞系。 我们获得了两个细胞系具有不同的形态，染色体和功能特性。 其中一种名为帕图8988 s的细胞在裸鼠异种移植物中显示出固体生长，细胞仅偶尔表现出细胞质的极性组织。 在一般情况下，没有顶端或基底侧质膜域可以区分和稀疏的细胞器随机分布在整个细胞质。 分泌产物，如粘蛋白，组织化学染色较弱或完全不存在。 在这些细胞中，用作细胞分化标志物的转氨酶（TGase）活性较低。 另一种名为帕图8988 t的细胞系，当皮下注射到裸鼠体内时，生长出由管状结构组成的肿瘤。 细胞具有明显的顶侧和底侧质膜，细胞质细胞器排列在细胞的下部，而顶端的细胞质含有高尔基复合体和许多分泌颗粒。 组织化学染色显示粘蛋白含量高，转氨酶活性比帕图8988 s高10倍。 比较每个中期平板的染色体数目，两种细胞系均显示出一个主峰，帕图8988 s每个中期平板有45-55条染色体，帕图8988 t每个中期平板约有110-120条染色体。 当将这两种细胞系静脉注射到裸鼠的尾静脉中时，仅帕图8988 s仅在肺中产生转移，而帕图8988 t在任何器官中均不产生转移。 我们的结论是，两个细胞系表现出不同的分化程度，以及不同的潜能转移，可以建立从相同的原发性肿瘤，这些细胞系代表了一个合适的模型，为进一步研究人类胰腺癌的细胞生物学。
From a liver metastasis of a human pancreatic adenocarcinoma, we have established cell lines for studying the cell biology of this tumor. We obtained two cell lines with different morphological, chromosomal and functional properties. One of them, named PaTu 8988s, revealed a solid growth in nude mouse xenografts with cells exhibiting only occasional polar organisation of the cytoplasm. In general, no apical or basolateral plasma membrane domains could be distinguished and the sparse organelles were randomly distributed throughout the cytoplasm. Secretory products, such as mucin, were weakly stained histochemically or were completely absent. Transglutaminase (TGase) activity used as a marker for cellular differentiation was low in these cells. The other cell line, named PaTu 8988t, grew tumors composed of tubular structures when injected subcutaneously into nude mice. Cells were polarized with distinct apical and basolateral plasma membranes and the cytoplasmatic organelles were arranged with the nucleus in the lower part of the cell, while the apical cytoplasm contained the Golgi complex and numerous secretion granules. A high content of mucin was stained histochemically and transglutaminase activity was ten times higher than in PaTu 8988s. Comparing the chromosome number per metaphase plate, both cell lines showed a major peak, with 45-55 chromosomes per metaphase plate in PaTu 8988s and about 110-120 chromosomes per metaphase plate in PaTu 8988t. When the two cell lines were injected intraveneously into the tail vein of nude mice, only PaTu 8988s developed metastases localized exclusively in the lung, whereas PaTu 8988t produced no metastases in any organ. We conclude, that two cell lines exhibiting different grades of differentiation as well as a different potency to metastasize can be established from the same primary tumor, and that these cell lines represent a suitable model for further study of the cell biology of human pancreatic adenocarcinoma.