High-Temperature Sintering of Xenogeneic Bone Substitutes Leads to Increased Multinucleated Giant Cell Formation: In Vivo and Preliminary Clinical Results
High-Temperature Sintering of Xenogeneic Bone Substitutes Leads to Increased Multinucleated Giant Cell Formation: In Vivo and Preliminary Clinical Results
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DOI：
10.1563/aaid-joi-d-14-00168
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发表时间：
2015-10-01
影响因子：
1.6
通讯作者：
Ghanaati, Shahram
中科院分区：
文献类型：
--
作者：
Barbeck, Mike;Udeabor, Samuel;Ghanaati, Shahram
本临床前和临床研究评估了对高温处理的异种材料（Bego-Oss）的炎症反应以及该材料对多核巨细胞发生、植入床血管化和再生潜力的影响。在通过扫描电子显微镜评价材料特性后，使用CD-1小鼠皮下植入来评估对材料长达60天的炎症反应。本研究的临床方面涉及使用上颌窦提升术后6个月的人骨标本。应用已建立的组织学和组织形态学分析方法。植入后，该材料很好地融入两个物种中，没有任何不良反应。在两个物种中均观察到材料诱导的多核巨细胞，并与血管化增强相关。这些结果表明，高温处理导致炎症组织对生物材料的反应增加，以及多核巨细胞形成的综合增加。需要进一步澄清多核巨细胞向所谓的破骨细胞样细胞或异物巨细胞的分化，以将这些细胞与材料的理化组成联系起来。
The present preclinical and clinical study assessed the inflammatory response to a high-temperature-treated xenogeneic material (Bego-Oss) and the effects of this material on the occurrence of multinucleated giant cells, implantation bed vascularization, and regenerative potential. After evaluation of the material characteristics via scanning electron microscopy, subcutaneous implantation in CD-1 mice was used to assess the inflammatory response to the material for up to 60 days. The clinical aspects of this study involved the use of human bone specimens 6 months after sinus augmentation. Established histologic and histomorphometric analysis methods were applied. After implantation, the material was well integrated into both species without any adverse reactions. Material-induced multinucleated giant cells were observed in both species and were associated with enhanced vascularization. These results revealed the high heat treatment led to an increase in the inflammatory tissue response to the biomaterial, and a combined increase in multinucleated giant cell formation. Further clarification of the differentiation of the multinucleated giant cells toward so-called osteoclast-like cells or foreign-body giant cells is needed to relate these cells to the physicochemical composition of the material.