EVIDENCE FOR A ROLE OF A VICINAL DITHIOL IN THE TRANSPORT OF GAMMA-BUTYROBETAINE IN AGROBACTERIUM SP
EVIDENCE FOR A ROLE OF A VICINAL DITHIOL IN THE TRANSPORT OF GAMMA-BUTYROBETAINE IN AGROBACTERIUM SP
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DOI：
10.1016/0300-9084(88)90013-2
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发表时间：
1988-10-01
期刊：
影响因子：
3.9
通讯作者：
DESHUSSES, J
中科院分区：
文献类型：
--
作者：
NOBILE, S;DESHUSSES, J
从土壤中分离出的一种农杆菌能够使用。-丁甜菜碱作为碳和氮的唯一来源。巯基参与γ的主动转运。采用巯基烷基化试剂n -乙基马来酰亚胺（NEM）和二巯基特异性试剂氧化苯larsin （PAO）对-丁甜菜碱进行了研究。两种试剂都能强烈抑制γ。-丁甜菜碱的摄取，但也诱导了积累的底物的释放，这表明运输系统要么含有二硫醇依赖蛋白，要么含有一个小的含硫醇分子参与了摄取现象。
An Agrobacterium sp. isolated from soil is able to use .gamma.-butyrobetaine as its sole source of carbon and nitrogen. The involvement of thiol groups for active transport of .gamma.-butyrobetaine was investigated by use of the thiol alkylating reagent N-ethylmaleimide (NEM) and the dithiol specific reagent phenylarsine oxide (PAO). Both reagents strongly inhibited .gamma.-butyrobetaine uptake, but also induced the release of the accumulated substrate, suggesting that the transport system either contains a dithiol-dependent protein or that a small thiol-containing molecule is implicated in the uptake phenomenon.