Administration of novel dyes for intraocular surgery: An in vivo toxicity animal study
Administration of novel dyes for intraocular surgery: An in vivo toxicity animal study
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DOI：
10.1167/iovs.06-0211
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发表时间：
2006-08-01
影响因子：
4.4
通讯作者：
Thaler, Sebastian
中科院分区：
文献类型：
--
作者：
Schuettauf, Frank;Haritoglou, Christos;Thaler, Sebastian
目的。为探讨新型活性染料玻璃体内注射对大鼠视网膜、视网膜色素上皮(RPE)和脉络膜的影响。方法玻璃体内注射4种染料：淡绿色SF黄(LGSF)、铜(II)酞菁四磺酸(E68)、溴酚蓝(BPB)和芝加哥蓝(CB)，分别溶解在浓度为0 5%和0 0 2%的生理盐水中。PSS作为对照。其他动物被单眼注射0.5%、0.02%、0.002%和0.0002%icg或0.002%E68。通过裂隙灯生物显微镜和检眼镜观察对前后节的不良反应。结果：用0.5%E68、0.5%ICG或0.5%CB处理的眼，在低浓度或其他染料下未见角膜和晶状体的离散染色。组织学显示E68给药后出现剂量依赖性反应。0.5%ICG组较PSS组视网膜内层明显变薄。0.02%的ICG导致7只眼中有3只眼的视网膜外部局灶性变性改变，而0.002%和0.0002%的ICG没有引起。CB导致了不同形态的改变。BPB或LGSF治疗眼显示正常的视网膜形态。ICG在所有受试浓度下均可引起明显的RGC丢失，而E68在0.5%浓度时也有明显的RGC丢失，但在较低浓度时不明显。结论：BPB或LGSF在体内对视网膜没有明显的毒性作用。这些新染料的安全性必须在其他模型和/或临床前研究中确定，然后才能临床使用。
PURPOSE. To investigate the effect of intravitreal injections of new vital dyes on the retina, the retinal pigment epithelium (RPE) and the choroid in an in vivo rat model.METHODS. Rats were injected intravitreally with four dyes: light-green SF yellowish (LGSF), copper(II) phthalocyanine-tetrasulfonic acid (E68), bromphenol blue (BPB), and Chicago blue ( CB) dissolved in physiologic saline solution (PSS) at concentrations of 0.5% and 0.02%. PSS served as the control. Additional animals were treated with single injections of 0.5%, 0.02%, 0.002%, and 0.0002% ICG or 0.002% E68 into one eye. Adverse effects on anterior and posterior segments were evaluated by slit lamp biomicroscopy and ophthalmoscopy. Retinal toxicity was assessed by histology and retinal ganglion cell (RGC) quantification 7 days after dye administration.RESULTS. Eyes treated with 0.5% E68, 0.5% ICG, or 0.5% CB showed discrete staining of both cornea and lens not seen at lower concentrations or with other dyes. Histology revealed dose-dependent reactions after E68 administration. ICG 0.5% induced significant thinning of inner retinal layers compared with PSS. ICG 0.02% caused focal degenerative changes of the outer retina in three of seven eyes, whereas 0.002% and 0.0002% ICG did not. CB led to heterogeneous morphologic alterations. BPB- or LGSF-treated eyes showed normal retinal morphology. ICG at all tested concentrations induced significant RGC loss, as did E68 at 0.5% but not at lower concentrations.CONCLUSIONS. BPB or LGSF produced no significantly detectable toxic effects on the retina in vivo. The safety of these new dyes must be established in other models and/or in preclinical studies before the clinical use of any of these dyes.