AVIDIN MONOMER AFFINITY COLUMN FOR THE PURIFICATION OF BIOTIN-CONTAINING ENZYMES
AVIDIN MONOMER AFFINITY COLUMN FOR THE PURIFICATION OF BIOTIN-CONTAINING ENZYMES
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DOI：
10.1016/0003-2697(79)90374-9
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
MALOY, WL
中科院分区：
文献类型：
--
作者：
HENRIKSON, KP;ALLEN, SHG;MALOY, WL
本文介绍了一种亲和素单体亲和柱的制备方法，该柱可用于含生物素酶的纯化。耻垢分枝杆菌的丙酰辅酶A羧化酶和丙酸蛛和谢氏丙酸杆菌[费氏]的甲基丙二酰辅酶A丙酮酸转羧酶（EC 2.1.3.1）均与色谱柱结合，并且可以用（+）-生物素特异性洗脱。
A procedure is given for the preparation of an avidin monomer affinity column which is useful in the purification of biotin-containing enzymes. Both the propionyl-CoA carboxylase of Mycobacterium smegmatis and the methylmalonyl-CoA pyruvate transcarboxylase (EC 2.1.3.1) of Arachnia propionica and Propionibacterium shermanii [freudenreichii] bind to the column and can be specifically eluted with (+)-biotin.