PROLIFERATIVE RESPONSE OF TAX1-TRANSDUCED PRIMARY HUMAN T-CELLS TO ANTI-CD3 ANTIBODY STIMULATION BY AN INTERLEUKIN-2-INDEPENDENT PATHWAY
PROLIFERATIVE RESPONSE OF TAX1-TRANSDUCED PRIMARY HUMAN T-CELLS TO ANTI-CD3 ANTIBODY STIMULATION BY AN INTERLEUKIN-2-INDEPENDENT PATHWAY
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DOI：
10.1128/jvi.67.3.1211-1217.1993
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发表时间：
1993-03-01
影响因子：
5.4
通讯作者：
SHIMOTOHNO, K
中科院分区：
文献类型：
--
作者：
AKAGI, T;SHIMOTOHNO, K
将人T细胞白血病病毒Tax 1转导的来源于外周血淋巴细胞的原代人T细胞的生长特性与用对照载体转导的相同T细胞亚群的生长特性进行比较。Tax 1转导的T细胞表现出轻微升高的反应，外部添加的白细胞介素-2（IL-2）和显着较高的增殖反应，抗CD 3抗体的刺激。抗CD 3抗体刺激后的增殖主要是通过IL-2非依赖性途径。因此，一些其他的机制比以前提出的IL-2自分泌模型似乎参与了由Tax 1的T细胞增殖的失调的过程。此外，Tax 1转导的T细胞在对照T细胞停止生长后很长时间内在含有IL-2的培养基中继续增殖，因此它们被认为是永生化的。
The growth properties of human T-cell leukemia virus Tax1-transduced primary human T cells derived from peripheral blood lymphocytes were compared with those of the same subset of T cells transduced with a control vector. Tax1-transduced T cells exhibited slightly elevated responsiveness to externally added interleukin-2 (IL-2) and a markedly higher proliferative response to stimulation with anti-CD3 antibody. The proliferation after anti-CD3 antibody stimulation was mainly via an IL-2-independent pathway. Therefore, some other mechanism than the previously proposed IL-2 autocrine model seems to be involved in the process of deregulation of T-cell proliferation by Tax1. Moreover, Tax1-transduced T cells have continued to proliferate in medium containing IL-2 long after control T cells ceased to grow, and so they are considered to be immortalized.