Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
复制标题
Kilo-sequencing：快速 DNA 序列数据采集的有序策略。
DOI：
10.1093/nar/11.2.349
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发表时间：
1983
影响因子：
14.9
通讯作者：
Bevan,M
中科院分区：
文献类型：
--
作者：
Barnes,WM;Bevan,M
A strategy for rapid DNA sequence acquisition in an ordered, nonrandom manner, while retaining all of the conveniences of the dideoxy method with M13 transducing phage DNA template, is described. Target DNA 3 to 14 kb in size can be stably carried by our M13 vectors. Suitable targets are stretches of DNA whichlackan enzyme recognition site which is unique on our cloning vectors and adjacent to the sequencing primer; current sites that are so useful when lacking are Pst, Xba, HindIII, BglII, EcoRI. By anin vitroprocedure, we cut RF DNA once randomly and once specifically, to create thousands of deletions which start at the unique restriction site adjacent to the dideoxy sequencing primer and extend various distances across the target DNA. Phage carrying a desired size of deletions, whose DNA as template will give rise to DNA sequence data in a desired location along the target DNA , may be purified by electophoresisaliveon agarose gels . Phage running in the same location on the agarose gel thus conveniently give rise to nucleotide sequence data from the same kilobase of target DNA.
