Low concentrations of interleukin-1 stimulate and high concentrations inhibit insulin release from isolated rat islets of Langerhans.
Low concentrations of interleukin-1 stimulate and high concentrations inhibit insulin release from isolated rat islets of Langerhans.
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DOI：
10.1530/acta.0.1130551
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发表时间：
1986-12
期刊：
影响因子：
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通讯作者：
G. A. Spinas;T. Mandrup-Poulsen;J. Mølvig;Leif Bæk;Klaus Bendtzen;C. Dinarello;J. Nerup
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文献类型：
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作者：
G. A. Spinas;T. Mandrup-Poulsen;J. Mølvig;Leif Bæk;Klaus Bendtzen;C. Dinarello;J. Nerup
将分离的大鼠胰岛分别与粗制、亲和纯化或重组人白介素1孵育1~6天。在所有三种含有白细胞介素1的制剂的低浓度下，胰岛素释放显著增加(20%-60%)。相反，高浓度则呈剂量依赖性地抑制胰岛素的释放。胰岛素分泌增加的浓度低于在体外增强小鼠胸腺细胞对植物血凝素的丝裂原反应所必需的浓度。这些剂量(0.05-0.5U/ml)相当于每毫升0.2-2 ng重组白介素1，约等于0.01-0.1pmol/ml。在0.6-1.8U/ml剂量下，亲和纯化的白介素1显著增加每ng DNA的胰岛胰岛素含量，表明刺激了胰岛素的生物合成。这些数据支持低浓度的白介素1可能在启动胰岛素的生理分泌方面发挥作用的概念。
Isolated rat islets were incubated either with crude, affinity-purified or recombinant human interleukin-1 for 1 to 6 days. A significant (20-60%) increase of insulin release was observed at low concentrations of all three interleukin-1-containing preparations. In contrast, higher concentrations dose-dependently inhibited the insulin release. The increased insulin secretion occurred at concentrations below those necessary to augment the mitogen response to phytohaemagglutinin of murine thymocytes in vitro. These doses (0.05-0.5 U/ml) correspond to 0.2-2 ng of recombinant interleukin-1 per ml, equal to approximately 0.01-0.1 pmol/ml. In doses of 0.6-1.8 U/ml affinity-purified interleukin-1 significantly increased the islet insulin content per ng of DNA, indicating a stimulation of insulin-biosynthesis. The data support the concept that low concentrations of interleukin-1 may play a role in priming the physiological secretion of insulin.