Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination
Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination
复制标题
DOI：
10.1016/j.theriogenology.2011.05.040
复制
发表时间：
2011-11-01
期刊：
影响因子：
2.8
通讯作者：
Gadella, B. M.
中科院分区：
文献类型：
--
作者：
Broekhuijse, M. L. W. J.;Sostaric, E.;Gadella, B. M.
为了获得更标准化的精液活力评估，Varkens KI Nederland在其所有猪人工智能实验室中引入了计算机辅助精液分析（CASA）系统。CASA的可重复性通过以下标准来提高：1)最佳样品温度（39℃）；2)最佳稀释系数；3)采用机械混合法优化精液与稀释缓冲液的混合；4)滑室的深度，并与前面的要点相结合；5) CASA系统技术人员的优化培训；6)标准操作程序（SOP）的使用。一旦实验室技术人员接受了使用该SOP的培训，他们的变异系数< 5%，优于未严格使用SOP时发现的变异系数。肉眼显微镜下精液活力评估是主观的，不能与标准化CASA获得的数据进行比较。CASA结果更可取，因为产生了准确的连续运动数据，而不是像实验室技术人员估计的那样，以10%的运动百分比增量进行区分。CASA发现的精子活力变异性较高，并且持续的活力值可以更好地分析精液活力特征与受精能力之间的关系。讨论了标准化CASA对人工授精（AI）剂量产生的射精的正确稀释系数（对于减少每次人工授精剂量的精子数量至关重要），从而从这些人工授精剂量中获得更可靠的生育数据。(C) 2011爱思唯尔公司版权所有。
In order to obtain a more standardised semen motility evaluation, Varkens KI Nederland has introduced a computer assisted semen analysis (CASA) system in all their pig AI laboratories. The repeatability of CASA was enhanced by standardising for: 1) an optimal sample temperature (39 degrees C); 2) an optimal dilution factor; 3) optimal mixing of semen and dilution buffer by using mechanical mixing; 4) the slide chamber depth, and together with the previous points; 5) the optimal training of technicians working with the CASA system; and 6) the use of a standard operating procedure (SOP). Once laboratory technicians were trained in using this SOP, they achieved a coefficient of variation of < 5% which was superior to the variation found when the SOP was not strictly used. Microscopic semen motility assessments by eye were subjective and not comparable to the data obtained by standardised CASA. CASA results are preferable as accurate continuous motility dates are generated rather than discrimination motility percentage increments of 10% motility as with motility estimation by laboratory technicians. The higher variability of sperm motility found with CASA and the continuous motility values allow better analysis of the relationship between semen motility characteristics and fertilising capacity. The benefits of standardised CASA for AI is discussed both with respect to estimate the correct dilution factor of the ejaculate for the production of artificial insemination (AI) doses (critical for reducing the number of sperm per AI doses) and thus to get more reliable fertility data from these AI doses in return. (C) 2011 Elsevier Inc. All rights reserved.