IDENTIFICATION OF AN ALLATOTROPIN FROM ADULT MANDUCA-SEXTA
IDENTIFICATION OF AN ALLATOTROPIN FROM ADULT MANDUCA-SEXTA
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DOI：
10.1126/science.243.4897.1481
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发表时间：
1989-03-17
期刊：
影响因子：
56.9
通讯作者：
KRAMER, SJ
中科院分区：
文献类型：
--
作者：
KATAOKA, H;TOSCHI, A;KRAMER, SJ
一种在体外强烈刺激咽侧体分泌保幼激素的肽（allatotropin）已从pharate成虫天蛾头提取物中纯化。已确定该13个残基的肽的一级结构：H-Gly-Phe-Lys-Asn-Val-Glu-Met-Met-Thr-Ala-Arg-Gly-Phe-NH 2。这种神经激素与其他生物体的任何已知神经肽都没有序列相似性。合成的allatotropin，以及截短片段，包括一个与五个氨基末端残基删除，显示在体外活性与天然allatotropin没有区别。
A peptide that strongly stimulates the secretion of juvenile hormone from corpora allata in vitro (allatotropin) has been purified from extracts of heads of pharate adult Manduca sexta. The primary structure of this 13-residue peptide has been determined: H-Gly-Phe-Lys-Asn-Val-Glu-Met-Met-Thr-Ala-Arg-Gly-Phe-NH2. This neurohormone has no sequence similarity with any known neuropeptide from other organisms. Synthetic allatotropin, as well as truncation fragments, including one with the five amino terminal residues deleted, showed in vitro activity indistinguishable from that of native allatotropin.