Use of radioyttrium to study food movement in the small intestine of the rat.
Use of radioyttrium to study food movement in the small intestine of the rat.
复制标题
使用放射性钇研究大鼠小肠中的食物运动。
DOI：
10.1093/jn/76.2.179
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发表时间：
1962
期刊：
影响因子：
--
通讯作者：
F. Lengemann
中科院分区：
文献类型：
--
作者：
C. Marcus;F. Lengemann
利用Y/sup 91/标记固体和液体食物的新方法，使计算食物通过大鼠小肠的速度成为可能。在pH值为3的食物样本中，约85%的Y/sup 91/与磨碎的食物结合在一起，随着pH值的增加，约96%的Y/sup 91/与pH值为8的食物结合在一起。口服Y/sup 91/的大鼠肾脏计数没有明显高于背景值。大鼠消化道Y/sup 91/的总回收率平均为97.9±0.8%。因此，Y/sup 91/不会被吸收，并与食物保持在一起，是食物位置的有效指标。在喂食后的预定时间间隔内杀死大鼠，并根据时间绘制消化道部分中Y/sup 91/剂量的百分比；然后将每条曲线分解为输入和输出分量。最高的通过率发生在近端小肠；然后，这一比率持续下降，直到在远端小肠中，它们下降了近20倍。这种不可吸收的放射性指示技术允许在不受干扰的消化道中进行体内实验，保留了自然的生理条件。液体和固体食物都可以使用，从而与正常饮食平行。这种方法似乎是可靠的，因为数据与计算值一致，而且它比在对准的转基因管下移动整个肠道的技术更简单、更准确。(H.H.D.)«更少
A new method, using Y/sup 91/ to label solid and liquid food, made it possible to calculate the rate of passage of food through the small intestine of rats. In food samples at pH 3, about 85% of the Y/sup 91/ stayed combined with the ground food and, as the pH increased, about 96% of the Y/sup 91/ was combined with the food at pH 8. Kidneys of rats given Y/sup 91/ orally showed no counts significantly above background. Total recovery of Y/sup 91/ in the digestive tracts of the rats averaged 97.9 plus or minus 0.8%. Thus Y/sup 91/ does not undergo absorption and by staying with the food is a valid indicator of food location. Rats were killed at predetermined time intervals after feeding, and the percentage of the Y/sup 91/ dose present in portions of the digestive tract was plotted against time for each alimentary segment; each curve was then resolved into input and outgo components. The highest rates of passage occurred in the proximal small gut; the rates then diminished continuously until, in the distal small intestine, they had decreased by a factor of almost 20. This nonabsorbable radio-indicator technique permitted in vivo experiments with undisturbedmore » alimentary tracts, preserving the naturai physiological conditions. Both liquid and solid food may be used, thus paralleling a normal diet. The method seems reliabie because the data agreed with calculated values, and it is simpier and more accurate than the technique in which the whole gut is moved under a collimated GM tube. (H.H.D.)« less