Characterization of tumor imaging with microbubble-based ultrasound contrast agent, Sonazoid, in rabbit liver
Characterization of tumor imaging with microbubble-based ultrasound contrast agent, Sonazoid, in rabbit liver
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DOI：
10.1248/bpb.28.972
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发表时间：
2005-06-01
影响因子：
2
通讯作者：
Fujimaki, M
中科院分区：
文献类型：
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作者：
Watanabe, R;Matsumura, M;Fujimaki, M
为了探讨Sonazoid相反转显像对肝脏肿瘤检漏率的提高作用，采用影像密度法和目测法评价了Sonazoid对转移性癌模型家兔肝脏的造影剂作用。使用相同的动物研究了索那唑类药物的对比增强与组织病理学之间的相关性。电镜观察另一只健康家兔肝组织中索那唑类微泡的分布。结果所有肿瘤直径均小于12mm，静脉注射Sonazoid后，肿瘤在早期（长达30s）被一环增强信号包围，随后在延迟期（10min后）出现明显的对比缺损。组织病理学观察显示，环形强化是由肿瘤内的新生血管引起的，对比缺损对应肿瘤的活区和死区，缺Kupffer细胞。在延迟期，肿瘤与健康组织的影像密度差异明显增加，使用索那唑类药物后，肿瘤的视觉检出率提高。超微结构分析显示，Kupffer细胞具有微泡样结构，表明索那唑类微泡被这些细胞所吸收。总之，Sonazoid与相位倒置成像一起使用，通过在早期突出新生血管，并在延迟期由于没有占用Sonazoid微泡的Kupffer细胞而提供清晰的造影剂缺陷，大大提高了小肝肿瘤的可检出性。
In order to investigate improvement of hepatic tumor detectability by Sonazoid with phase inversion imaging, the contrast effects on the liver of metastatic carcinoma-model rabbits were evaluated by videodensitometry and visual assessment. Correlation between the contrast enhancement of Sonazoid and histopathology was examined using the same animals. Electron microscopy was performed on hepatic tissue from another healthy rabbits to identify the distribution of Sonazoid microbubbles. As a result, all tumors were smaller than 12 mm in diameter, and after intravenous injection of Sonazoid, they were surrounded with a ring of enhanced signal during the early phase (up to 30 s), followed by a clear contrast defect during the delayed phase (after 10 min). Histopathologic observation revealed that the ring-enhancement was caused by neovasculature in the tumor, and the contrast defects corresponded to living and dead parts of tumors, which lack Kupffer cells. Videodensitometric differences between tumor and healthy tissue markedly increased at delayed phase, and visual detectability of tumors was improved when Sonazoid was used. Ultrastructural analysis showed microbubble-like structures in Kupffer cells, which indicated that Sonazoid microbubbles were taken up with these cells. In conclusion, Sonazoid, used with phase inversion imaging, greatly increases the detectability of small hepatic tumors by highlighting neovascularity at early phase and providing clear contrast defects due to absence of Kupffer cells, which take up Sonazoid microbubbles, at delayed phase.