A novel chromatographic method for the preparation of high density lipoproteins.
A novel chromatographic method for the preparation of high density lipoproteins.
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一种制备高密度脂蛋白的新色谱方法。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
A. Lacko
中科院分区：
文献类型：
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作者：
M. Jahani;R. G. Huttash;A. Lacko
高密度脂蛋白（HDL）通过采用聚阴离子沉淀和柱层析的程序进行分离。产物聚阴离子沉淀并柱层析。该产品不含低密度脂蛋白（LDL），但仍然存在血清白蛋白（HSA）。通过免疫吸附柱除去剩余的HSA。通过我们的方法分离的 HDL 与通过超速离心和凝胶色谱相结合从同一血浆样品中分离出的另一种 HDL 制剂进行比较。发现它具有与通过常规技术分离的HDL大致相同的脂质和蛋白质组成。较小的差异包括通过柱色谱分离的 HDL 的磷脂和脱辅基蛋白 E 含量较高，甘油三酯和 ApoC II 含量较低。这里描述的方法比早期技术要简单得多，可以在不大幅增加劳动力的情况下扩大规模，并且产量比 Rudel 等人描述的方法高约 30%。
High density lipoproteins (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. The product polyanion precipitation and column chromatography. The product was free of low denisty lipoproteins (LDL) but serum albumin (HSA) was still present. The remaining HSA was removed by an immunoadsorbent column. The HDL isolated by our method was compared to another HDL preparation isolated from the same plasma sample by the combination of ultracentrifugation and gel chromatgraphy. It was found to have approximately the same lipid and protein composition as the HDL isolated by conventional techniques. Minor differences included a higher phospholipid and apoprotein E content and lower triglyceride and ApoC II content of the HDL isolated by column chromatography. The method described here is considerably less tedious than earlier techniques, can be scaled up without substantial increase in labor and results in an approximately 30% higher yield than the method described by Rudel et al.