Cyclic nucleotide-gated channel activation is not required for activity-dependent labeling of zebrafish olfactory receptor neurons by amino acids.
Cyclic nucleotide-gated channel activation is not required for activity-dependent labeling of zebrafish olfactory receptor neurons by amino acids.
复制标题
斑马鱼嗅觉受体神经元的氨基酸活性依赖性标记不需要环状核苷酸门控通道激活。
DOI：
10.1159/000014607
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Michel,WC
中科院分区：
文献类型：
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作者：
Michel,WC
鱼类的嗅觉上皮在受体细胞 (ORN) 类型和这些细胞中表达的气味受体家族方面都是异质的。由于这种多样性，由气味剂激活的转导级联尚未明确建立。在当前的研究中，使用电生理学和活性依赖性标记技术来评估环核苷酸门控通道在斑马鱼嗅觉转导中的作用。氨基酸和胆汁盐气味剂均引起强烈的电生理反应，然而，ORN 的活性依赖性标记只能由氨基酸气味剂刺激。腺苷酸环化酶 (AC) 激活剂（毛喉素）和磷酸二酯酶抑制剂（3-异丁基-1-甲基黄嘌呤，IBMX）也引发了强烈的电生理反应；通常大于氨基酸或胆汁盐气味剂引起的反应。然而，单独的毛喉素或毛喉素和 IBMX 的混合物都不会刺激活性依赖性标记。用毛喉素浸泡嗅觉上皮，这可能增加了细胞内 cAMP 的浓度，与氨基酸气味剂相比，显着更大程度地减少了对胆汁盐气味剂的反应。总的来说，这些发现表明氨基酸输入的转导并不主要依赖于环核苷酸门控（CNG）通道的激活，并且CNG通道激活可能是胆汁盐输入转导所必需的。
The olfactory epithelium of fish is heterogeneous both with respect to the types of receptor cells (ORNs) present and the families of odorant receptors expressed in these cells. As a consequence of this diversity, the transduction cascade(s) activated by odorants has yet to be unambiguously established. In the current study, electrophysiological and activity-dependent labeling techniques were used to assess the role of the cyclic nucleotide-gated channel in zebrafish olfactory transduction. Both amino acid and bile salt odorants elicited robust electrophysiological responses, however, activity-dependent labeling of ORNs could be stimulated only by the amino acid odorants. An adenylate cyclase (AC) activator (forskolin) and a phosphodiesterase inhibitor (3-isobutyl-1-methylxanthine, IBMX) also elicited robust electrophysiological responses; generally larger than the responses elicited by either the amino acid or bile salt odorants. However, neither forskolin alone or a mixture of forskolin and IBMX stimulated activity-dependent labeling. Bathing the olfactory epithelium with forskolin, which presumably increased the intracellular concentration of cAMP, reduced the responses to bile salt odorants to a significantly greater extent than amino acid odorants. Collectively, these findings suggest that the transduction of amino acid input does not rely primarily on cyclic nucleotide-gated (CNG) channel activation and that CNG channel activation may be required for the transduction of bile salt input.