Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells
Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells
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DOI：
10.1016/j.rbmo.2015.09.011
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发表时间：
2016-01-01
影响因子：
4
通讯作者：
Akoum, Ali
中科院分区：
文献类型：
--
作者：
Bourdiec, Amelie;Ahmad, Syed-Furquan;Akoum, Ali
白细胞介素（IL） 1家族成员在子宫内膜接受胚胎的准备过程中的表达机制尚不清楚。在本研究中，去个体化使IL1家族受体的表达失衡，其模式增加了子宫内膜基质细胞对IL1、IL18和IL33的接受性。此外，子宫内膜细胞显示同源盒HOXA10、HOXA11和LIFR的表达增加，这是已知参与子宫内膜胚胎接受性的基因。对子宫内膜蜕膜细胞的进一步分析显示，与胚胎侵袭过程相关的有效促炎、重构和血管生成因子的释放显著增加，如VEGF （P = 0.0305）、MMP9 （P = 0.0003）、TIMP3 （P = 0.0001）、RANTES （P = 0.0020）、MCP1 （P = 0.0001）和MIF （P = 0.0068）。内源性il - 1b分泌未见明显变化。il - 18分泌减少，去个体化使il - 33分泌增加。这些发现揭示了在子宫内膜基质细胞脱个体化过程中，子宫内膜细胞接受性对il - 1家族成员的显著调节，并提示il - 1家族成员的参与在子宫内膜接受性的生理过程中很重要，包括适应性免疫。这可能与建立一个有利于胚胎着床的子宫微环境有关。(C) 2015生殖保健有限公司Elsevier Ltd.出版。版权所有。
The mechanisms involving the expression of interleukin (IL) 1 family members in the process of preparing the endometrium to receive an embryo remain unclear. In this study, decidualization differentially skewed the balance of IL1 family receptor expression in a pattern that increases endometrial stromal cell receptivity to IL1, IL18 and IL33. Additionally, endometrial cells showed increased expression of homeobox HOXA10 and HOXA11 and LIFR, which are known to be involved in endometrial embryo receptivity. Further analyses of decidual endometrial cells revealed a significant increase in the release of potent proinflammatory, remodelling and angiogenic factors implicated in the embryo invasion process, such as VEGF (P = 0.0305), MMP9 (P = 0.0003), TIMP3 (P = 0.0001), RANTES (P = 0.0020), MCP1 (P = 0.0001) and MIF (P = 0.0068). No significant changes in endogenous IL1B secretion were observed. Decreased secretion of IL18 and decidualization increased secretion of IL33. These findings reveal a significant modulation of endometrial cell receptivity to IL1 family members during endometrial stromal cell decidualization, and suggest that the involvement of IL1 family members is important in physiological processes of endometrial receptivity, including adaptive immunology. This may be relevant to establishing a favourable uterine microenvironment for embryo implantation. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.