INHIBITION OF LECTIN-INDUCED LYMPHOCYTE-ACTIVATION BY DIAMIDE AND OTHER SULFHYDRYL-REAGENTS
INHIBITION OF LECTIN-INDUCED LYMPHOCYTE-ACTIVATION BY DIAMIDE AND OTHER SULFHYDRYL-REAGENTS
复制标题
DOI：
10.1016/0008-8749(78)90274-5
复制
发表时间：
1978-01-01
影响因子：
4.3
通讯作者：
WEDNER, HJ
中科院分区：
文献类型：
--
作者：
CHAPLIN, DD;WEDNER, HJ
Inhibition of lectin-induced [human] lymphocyte activation by 5 reagents capable of combining with or oxidizing free sulfhydryl groups was examined. Each of the reagents tested was capable of inhibiting [methyl-3H]thymidine or [14C]uridine incorporation into trichloroacetic acid-insoluble material. Four of these reagents, iodoacetamide and N-ethylmaleimide (alkylating agents) and 5,5''-dithiobis (2-nitrobenzoic acid) and p-hydroxymercuriphenylsulfonic acid (sulfhydryl binding agents), inhibited activation when added to lymphocyte cultures together with lectin or at any time thereafter through 48 h. The sulfhydryl oxidizing agent diazine dicarboxylic acid bis[N,N-dimethylamide] (diamide) was effective only when added within 30-60 min of lectin or when added after 24 h. This inhibition of lymphocyte activation was not due to decreased intracellular levels of reduced glutathione or to inhibition of binding of lectin to the lymphocyte. Maintenance of free sulfhydryl groups may be important during the early induction of lymphocyte activation, and an obligatory step or steps in the activation sequence may involve sulfhydryl interactions.
