Time Course of SERCA 2b and Calreticulin Expression in Purkinje Neurons of Ethanol-Fed Rats with Behavioral Correlates
Time Course of SERCA 2b and Calreticulin Expression in Purkinje Neurons of Ethanol-Fed Rats with Behavioral Correlates
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DOI：
10.1093/alcalc/agt062
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发表时间：
2013-11-01
影响因子：
2.8
通讯作者：
Dlugos, Cynthia A.
中科院分区：
文献类型：
--
作者：
Cassidy, Linda L.;Dlugos, Frederick F.;Dlugos, Cynthia A.
成年大鼠长期摄入乙醇 40 周会导致广泛的平滑内质网 (SER) 扩张，SER 是浦肯野神经元 (PN) 树突内钙稳态系统的主要组成部分。目的：本研究的目的是确定长期乙醇消耗是否会导致 PN 树突 SER 膜上的肌浆/内质网 Ca2+ ATP 酶泵 (SERCA) 发生改变。还研究了 PN 中钙网蛋白（一种钙伴侣）的密度以及平衡能力。方法：90 只 8 个月大的大鼠接受大鼠饲料、AIN-93 M 液体对照或乙醇饮食（30 只/饮食），持续 10、20 或 40 周（30 周/持续时间）。用 3 个月大的对照大鼠 (n = 10) 评估相对于大鼠饲料对照的年龄变化。在安乐死之前评估平衡。使用定量免疫细胞化学测定 SERCA 2b + 树突和钙网蛋白 + PN 胞体和细胞核的密度。还测定了分子层体积。结果：经过 40 周的乙醇处理，乙醇导致树突状乔木内的 SERCA 2b 密度下降，并且在更困难的圆杆平衡测试中平衡能力下降。没有乙醇引起的钙网蛋白密度变化。结论：可以得出结论，乙醇诱导的 SERCA 泵减少伴随着 SER 扩张，并导致先前报道的乙醇诱导的 PN 树突状退化。乙醇引起的平衡变化也发生了。慢性乙醇消耗不会改变 PN 中钙网蛋白的表达。
Chronic ethanol consumption for 40 weeks in adult rats results in dilation of the extensive smooth endoplasmic reticulum (SER), a major component of the calcium homeostatic system within Purkinje neuron (PN) dendrites. Aims: The aim of the present study was to determine whether chronic ethanol consumption results in alterations of the sarco/endoplasmic reticulum Ca2+ ATPase pump (SERCA) on the SER membrane of PN dendrites. The density of calreticulin, a calcium chaperone, was also investigated in the PN along with balancing ability. Methods: Ninety 8-month-old rats were exposed to rat chow, the AIN-93 M liquid control or ethanol diets (30/diet) for a duration of 10, 20 or 40 weeks (30/duration). Age changes relative to the rat chow controls were assessed with 3-month-old control rats (n = 10). Balance was assessed prior to euthanasia. Quantitative immunocytochemistry was used to determine the density of SERCA 2b + dendrites and calreticulin + PN soma and nuclei. Molecular layer volumes were also determined. Results: Following 40 weeks of ethanol treatment, there were ethanol-induced decreases in SERCA 2b densities within the dendritic arbor and decreased balancing ability on the more difficult round rod balance test. There were no ethanol-induced changes in calreticulin densities. Conclusion: It can be concluded that ethanol-induced decreases in the SERCA pump accompany SER dilation and contribute to previously reported ethanol-induced dendritic regression in PN. Ethanol-induced changes in balance also occurred. Chronic ethanol consumption does not alter calreticulin expression in PN.