Combined microbial community level and single species biosensor responses to monitor recovery of oil polluted soil
Combined microbial community level and single species biosensor responses to monitor recovery of oil polluted soil
复制标题
DOI：
10.1016/j.soilbio.2004.02.025
复制
发表时间：
2004-07-01
影响因子：
9.7
通讯作者：
Campbell, CD
中科院分区：
文献类型：
--
作者：
Bundy, JG;Paton, GI;Campbell, CD
要了解石油污染对土壤微生物群组成和功能的影响，就需要在复杂的环境基质中对群落水平上碳氢化合物混合物的影响进行调查。解决这一难题的一种方法是将社区水平的指纹识别方法与具有生理或功能意义的生物测定方法结合起来。采用两种对照成品油（石蜡油和机油）污染土壤微生物，并采用添加营养物的模拟生物修复处理。利用互补的群落水平技术(利用Biolog（R)微孔板进行碳源生理分析和磷脂脂肪酸（PLFA）分析）对本地微生物进行了103 d的监测。使用发光细菌生物测定法监测所涂油的毒性变化，包括菲氏弧菌和一种降解碳氢化合物的恶臭假单胞菌菌株。由于油类污染，微生物群落结构和碳源利用谱发生了明显变化。有一些证据表明，生物修复土壤在实验结束时恢复到控制值。这一点得到了生物测定结果的支持，该结果显示，由于添加了油，最初毒性有所增加，但在实验结束时毒性有所下降。两种精油对生物试验生物的毒性表现出明显的差异，且具有物种特异性差异。在PLFA剖面中也发现了这种油特异性差异，这表明两种油类型选择了不同的微生物群落。(C) 2004 Elsevier Ltd.版权所有。
Understanding the effects of oil contamination on the composition and function of soil microbiota entails investigation of the effects of a mixture of hydrocarbons at the community level in a complex environmental matrix. One approach to this difficult problem is to ally a community-level fingerprinting approach with bioassays that have a physiological or functional implication. Two contrasting refined oils (paraffin and motor oil) were used to contaminate soil microcosms, and a simulated bioremediation treatment with nutrient-addition was applied. The indigenous microorganisms were monitored over 103 d using complementary community-level techniques (carbon source physiological profiling using Biolog(R) microplates, and phospholipid fatty acid (PLFA) profiling). Changes in the toxicity of the applied oils were monitored using luminescent bacterial bioassays, including Vibriofischeri and a hydrocarbon-degrading Pseudomonas putida strain. Distinct shifts in microbial community structure and C source utilization profiles were observed as a result of oil contamination. There was some evidence that bioremediated soils were returning to control values by the end of the experiment. This was supported by the bioassay results which showed an initial increase in toxicity as a result of the oil addition which had then decreased by the conclusion of the experiment. The two oils exhibited markedly different toxicity towards the bioassay organisms, with species-specific differences in response. This oil-specific difference was also found in the PLFA profiles which showed the two oil types selected different microbial communities. (C) 2004 Elsevier Ltd. All rights reserved.