TUMOR-SUPPRESSOR ACTIVITY OF H19 RNA
TUMOR-SUPPRESSOR ACTIVITY OF H19 RNA
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DOI：
10.1038/365764a0
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发表时间：
1993-10-21
期刊：
影响因子：
64.8
通讯作者：
TYCKO, B
中科院分区：
文献类型：
--
作者：
HAO, Y;CRENSHAW, T;TYCKO, B
在某些人类胚胎肿瘤中，杂合性的缺失与染色体11p15.5上的肿瘤抑制基因有关，而母体等位基因的选择性缺失表明该基因是父系印记的1-4。人类H19基因定位于11p15.5，在分化的胎儿细胞中表达5-11，并且是父系印记12-16。我们在此报告了两种胚胎肿瘤细胞系RD和G401在转染H19表达构建体后表现出生长迟缓和形态变化。更重要的是，G401-H19在软琼脂中的克隆原性和裸鼠的致瘤性均被消除。除了证明其肿瘤抑制潜能外，这种转染系统应该有助于神秘的H19基因的结构和功能研究。
Loss of heterozygosity in certain human embryonal tumours implicates a tumour-suppressor gene at chromosome 11p15.5 and selective loss of maternal alleles suggests that this gene is paternally imprinted1-4. The human H19 gene maps to 11p15.5, is expressed in differentiating fetal cells5-11 and is paternally imprinted12-16. We report here that two embryonal tumour cell lines, RD and G401, showed growth retardation and morphological changes when transfected with an H19 expression construct. More importantly, clonogenicity in soft agar and tumorigenicity in nude mice were abrogated in the G401-H19 transfectants. In addition to demonstrating its tumour-suppressor potential, this transfection system should help structural and functional studies of the enigmatic H19 gene.