Early changes in sperm motility, acrosome reaction, and gene expression of reproductive organs in rats treated with sulfasalazine
Early changes in sperm motility, acrosome reaction, and gene expression of reproductive organs in rats treated with sulfasalazine
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DOI：
10.1016/j.reprotox.2006.10.003
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发表时间：
2007-02-01
影响因子：
3.3
通讯作者：
Horii, Ikuo
中科院分区：
文献类型：
--
作者：
Fukushima, Tamio;Hamada, Yoshimasa;Horii, Ikuo
以前，我们报道了CD59和衰变加速因子（decay accelerating factor，DIF）基因的附睾表达降低可能会影响长期（28天）给予柳氮磺胺吡啶的大鼠的精子活力和顶体反应。为了研究柳氮磺胺吡啶对雄性生殖道的早期影响，我们目前检查了用柳氮磺胺吡啶处理1、7或14天的大鼠的精子活力、顶体反应和睾丸和附睾中的基因表达。在第7天观察到精子活力降低和顶体反应，然而，睾丸基因表达没有显著变化。另一方面，早在第1天就观察到CD59和CD49的附睾基因表达减弱。由于CD59和CD40由附睾分泌并在精子成熟中发挥作用，我们假设柳氮磺胺吡啶影响精子成熟是一种早期效应，CD59和CD40基因与负面效应有关。(c)2006年爱思唯尔公司All rights reserved.
Previously, we reported that decreased epididymal expression of CD59 and decay accelerating factor (DAF) genes may affect sperm motility and the acrosome reaction in rats treated long-term (28 days) with sulfasalazine. To investigate the early effects of sulfasalazine on the male reproductive tract, we presently examined sperm motility, the acrosome reaction, and gene expression in the testes and epididymides of rats treated with sulfasalazine for 1, 7 or 14 days. Reduced sperm motility and acrosome reactions were noted on day 7, however, there were no remarkable changes in testicular gene expression. On the other hand, attenuated epididymal gene expression of CD59 and DAF was observed as early as day 1. As CD59 and DAF are secreted from the epididymis and play a role in sperm maturation, we hypothesize that sulfasalazine affects sperm maturation as an early effect and that CD59 and DAF genes are related to the negative effect. (c) 2006 Elsevier Inc. All rights reserved.