Skin sensitization potency of isoeugenol and its dimers evaluated by a non‐radioisotopic modification of the local lymph node assay and guinea pig maximization test
Skin sensitization potency of isoeugenol and its dimers evaluated by a non‐radioisotopic modification of the local lymph node assay and guinea pig maximization test
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通过局部淋巴结试验和豚鼠最大化试验的非放射性同位素改良评估异香酚及其二聚体的皮肤致敏效力
DOI：
10.1002/jat.1305
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发表时间：
2008
影响因子：
3.3
通讯作者：
S. Yamazaki
中科院分区：
文献类型：
--
作者：
M. Takeyoshi;K. Iida;Keiko Suzuki;S. Yamazaki
过敏性接触性皮炎是由使用化妆品等消费品引起的严重不良反应。异丁香酚是一种香料化学品，具有辛辣、康乃馨般的香味，用于多种化妆品中，是一种众所周知的中度人类致敏剂。据以前报道，丁香酚的二聚体产生两种类型的二聚体，具有不同的敏化效力。本研究报告了通过非放射性同位素局部淋巴结试验（非RI LLNA）和豚鼠最大剂量试验评价的异丁香酚和两种二聚体β-O-4-二木质素和脱氢二异丁香酚（DIEG）的皮肤致敏效力差异。在豚鼠最大剂量致敏试验中，异丁香酚、β-O-4-二木质素和DIEG分别被归类为极、弱和中度致敏剂。对于非RI LLNA的结果，异丁香酚、β-O-4-二木质素和DIEG的EC 3分别计算为12.7%、>30%和9.4%。这两种类型的异丁香酚二聚体表现出不同的致敏活性，类似于丁香酚二聚体的情况。通过二聚化实现的致敏效力的降低可能导致开发更安全的化妆品成分。异丁香酚二聚体目前不用于香料化学品。然而，异丁香酚的二聚化可能产生作为具有低致敏风险的化妆品成分的有希望的候选物。这些数据也可以为皮肤致敏的构效关系（SAR）提供有用的信息。版权所有© 2007约翰威利父子有限公司。
Allergic contact dermatitis is the serious unwanted effect arising from the use of consumer products such as cosmetics. Isoeugenol is a fragrance chemical with spicy, carnation‐like scent, is used in many kinds of cosmetics and is a well‐known moderate human sensitizer. It was previously reported that the dimerization of eugenol yielded two types of dimer possessing different sensitization potencies. This study reports the differences in skin sensitization potencies for isoeugenol and two types of dimer, β‐O‐4‐dilignol and dehydrodiisoeugenol (DIEG), as evaluated by the non‐radioisotopic local lymph node assay (non‐RI LLNA) and guinea pig maximization test. In the guinea pig maximization test, isoeugenol, β‐O‐4‐dilignol and DIEG were classified as extreme, weak and moderate sensitizers, respectively. As for the results of non‐RI LLNA, the EC3 for isoeugenol, β‐O‐4‐dilignol and DIEG were calculated as 12.7%, >30% and 9.4%, respectively. The two types of isoeugenol dimer showed different sensitizing activities similar to the case for eugenol dimers. A reduction of sensitization potency achieved by dimerization may lead to developing safer cosmetic ingredients. Isoeugenol dimers are not currently used for fragrance chemicals. However, the dimerization of isoeugenol may yield a promising candidate as a cosmetic ingredient with low sensitization risk. The data may also provide useful information for the structure‐activity relationship (SAR) in skin sensitization. Copyright © 2007 John Wiley & Sons, Ltd.