Three-Dimensional Mapping of Chorioretinal Vascular Oxygen Tension in the Rat
Three-Dimensional Mapping of Chorioretinal Vascular Oxygen Tension in the Rat
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DOI：
10.1167/iovs.08-2343
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发表时间：
2009-02-01
影响因子：
4.4
通讯作者：
Mori, Marek
中科院分区：
文献类型：
--
作者：
Shahidi, Mahnaz;Wanek, Justin;Mori, Marek
目的。开发了光学切片磷光寿命成像系统，用于脉络膜视网膜脉管系统中氧张力（Po-2）的三维绘图。方法。静脉注射氧敏感分子探针后，以斜角投射激光线并在视网膜上扫描，生成磷光光学切片图像。自动化软件算法对空间相邻位置的图像进行分割和组合，以构建深度位移的正面视网膜图像。通过确定磷光寿命来测量血管内Po-2。在不同吸入氧比例下，在大鼠眼中生成三维脉络膜视网膜 Po-2 图。结果。在空气呼吸条件下，脉络膜、视网膜动脉、毛细血管和静脉的平均Po-2分别为58+/-2mmHg、47+/-2mmHg、44+/-2mmHg和35+/-2mmHg。平均动静脉 Po-2 差异为 12 +/- 2 mm Hg。与空气呼吸条件下的测量相比，吸入氧分数较低时，脉络膜视网膜血管 Po-2 和平均动静脉 Po-2 差异降低。视网膜静脉 Po-2 在统计学上低于在视网膜动脉、毛细血管和脉络膜中测量的 Po-2 (P < 0.004)。结论。脉络膜视网膜氧张力的三维绘图可以对大视网膜血管和视网膜毛细血管中的 Po-2 进行定量测量。这种方法有可能有助于更好地了解健康和疾病中的视网膜氧合。 （投资眼科可见科学。2009；50：820-825）DOI：10.1167/iovs.08-2343
PURPOSE. An optical section phosphorescence lifetime imaging system was developed for three-dimensional mapping of oxygen tension (Po-2) in chorioretinal vasculatures.METHODS. A laser line was projected at an oblique angle and scanned on the retina after intravenous injection of an oxygen-sensitive molecular probe to generate phosphorescence optical section images. An automated software algorithm segmented and combined images from spatially adjacent locations to construct depth-displaced en face retinal images. Intravascular Po-2 was measured by determining the phosphorescence lifetime. Three-dimensional chorioretinal Po-2 maps were generated in rat eyes under varying fractions of inspired oxygen.RESULTS. Under an air-breathing condition, mean Po-2 in the choroid, retinal arteries, capillaries, and veins were 58 +/- 2 mm Hg, 47 +/- 2 mm Hg, 44 +/- 2 mm Hg, and 35 +/- 2 mm Hg, respectively. The mean arteriovenous Po-2 difference was 12 +/- 2 mm Hg. With a lower fraction of inspired oxygen, chorioretinal vascular Po-2 and mean arteriovenous Po-2 differences decreased compared with measurements under an air-breathing condition. Retinal venous Po-2 was statistically lower than Po-2 measured in the retinal artery, capillaries, and choroid (P < 0.004).CONCLUSIONS. Three-dimensional mapping of chorioretinal oxygen tension allowed quantitative Po-2 measurements in large retinal blood vessels and in retinal capillaries. This method has the potential to facilitate better understanding of retinal oxygenation in health and disease. (Invest Ophthalmol Vis Sci. 2009;50:820-825) DOI:10.1167/iovs.08-2343