Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules
Microfabricated airflow nozzle for microencapsulation of living cells into 150 micrometer microcapsules
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DOI：
10.1007/s10544-006-9011-9
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发表时间：
2007-01
影响因子：
2.8
通讯作者：
S. Sugiura;T. Oda;Y. Aoyagi;R. Matsuo;Tsuyoshi Enomoto;Kunio Matsumoto;Toshikazu Nakamura;Mitsuo Satake;Atsushi Ochiai;N. Ohkohchi;M. Nakajima
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文献类型：
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作者：
S. Sugiura;T. Oda;Y. Aoyagi;R. Matsuo;Tsuyoshi Enomoto;Kunio Matsumoto;Toshikazu Nakamura;Mitsuo Satake;Atsushi Ochiai;N. Ohkohchi;M. Nakajima
基因工程细胞的微囊化作为基因治疗的替代非病毒策略引起了广泛关注。虽然较小的微胶囊（即小于300 μm）理论上具有各种优势，但技术限制使其难以证明这一概念。我们开发了一种新型的微加工装置，即微气流喷嘴（MAN），以生产100至300 μm的海藻酸盐微胶囊，具有窄的粒径分布。MAN由用于海藻酸盐溶液通道的内径为60 μm的喷嘴和喷嘴旁边的气流通道组成。通过喷嘴挤出的藻酸盐溶液被气流剪切。产生的藻酸盐液滴直接落入CaCl2溶液中，形成藻酸钙珠。该设备使我们能够成功地将活细胞封装到150 μm的微胶囊中，并通过简单地改变气流速度来控制微胶囊的大小。与常规方法制备的较大微囊相比，150 μm微囊中的包封细胞具有更高的生长速率和更大的标记蛋白分泌活性，这是由于其高扩散效率和有效支架表面积。微胶囊的优点为微胶囊技术的发展提供了新的前景。
Microencapsulation of genetically engineered cells has attracted much attention as an alternative nonviral strategy to gene therapy. Though smaller microcapsules (i.e. less than 300 μm) theoretically have various advantages, technical limitations made it difficult to prove this notion. We have developed a novel microfabricated device, namely a micro-airflow-nozzle (MAN), to produce 100 to 300 μm alginate microcapsules with a narrow size distribution. The MAN is composed of a nozzle with a 60 μm internal diameter for an alginate solution channel and airflow channels next to the nozzle. An alginate solution extruded through the nozzle was sheared by the airflow. The resulting alginate droplets fell directly into a CaCl2solution, and calcium alginate beads were formed. The device enabled us to successfully encapsulate living cells into 150 μm microcapsules, as well as control microcapsule size by simply changing the airflow rate. The encapsulated cells had a higher growth rate and greater secretion activity of marker protein in 150 μm microcapsules compared to larger microcapsules prepared by conventional methods because of their high diffusion efficiency and effective scaffold surface area. The advantages of smaller microcapsules offer new prospects for the advancement of microencapsulation technology.