The three influenza virus polymerase (P) proteins not associated with viral nucleocapsids in the infected cell are in the form of a complex
The three influenza virus polymerase (P) proteins not associated with viral nucleocapsids in the infected cell are in the form of a complex
复制标题
与受感染细胞中的病毒核衣壳不相关的三种流感病毒聚合酶（P）蛋白呈复合物形式
作者：
B. Detjen;Carol ST. Angelo;M. Katze;R. M. Krug
与病毒核衣壳相关并负责病毒mRNA合成的三种流感病毒聚合酶或P蛋白（PB1， PB2和PA）在转录过程中以复合物的形式与生长的mRNA一起向下移动模板（J. Braam， I. Ulmanen, and R.M. Krug, Cell 34:609-618, 1983）。我们确定被感染的细胞是否包含一个与病毒核衣壳无关的P蛋白池，如果是这样，这个池中的P蛋白是否以复合物的形式相互结合。将感染细胞的细胞质和细胞核提取物离心去除核衣壳，用PB1蛋白或PB2蛋白特异性抗血清免疫沉淀得到上清。两种抗血清都沉淀了所有三种P蛋白，表明P蛋白处于一个复合物中，该复合物在很大程度上抵抗免疫沉淀缓冲液中存在的洗涤剂的破坏。蔗糖密度梯度分析表明，P蛋白复合物在11S ~ 22S范围内，在感染后1 h （2.5 ~ 3.5 h）内合成的PB1和PB2蛋白分子几乎都在这些复合物中。微量或未检测到游离PB1或PB2蛋白。讨论了这些非核衣壳P蛋白复合物在病毒特异性RNA合成的起始和再起始中的可能作用。
The three influenza virus polymerase, or P, proteins (PB1, PB2, and PA) that are associated with viral nucleocapsids and are responsible for viral mRNA synthesis are in the form of a complex that moves down the template in association with the growing mRNAs during transcription (J. Braam, I. Ulmanen, and R.M. Krug, Cell 34:609-618, 1983). We determined whether infected cells contained a pool of P proteins not associated with viral nucleocapsids and, if so, whether the P proteins in this pool were in the form of a complex with each other. The cytoplasmic and nuclear extracts from infected cells were depleted of nucleocapsids by centrifugation, and the resulting supernatants were subjected to immunoprecipitation with an antiserum specific for either the PB1 protein or the PB2 protein. Both antisera precipitated all three P proteins, indicating that the P proteins were in a complex that was largely resistant to disruption by the detergents present in the immunoprecipitation buffer. Sucrose density gradient analysis showed that the P protein complexes ranged from about 11S to 22S and that almost all of the PB1 and PB2 protein molecules synthesized during a 1-h period (2.5 to 3.5 h postinfection) were in these complexes. Little or no free PB1 or PB2 protein was detected. The possible role of these nonnucleocapsid P protein complexes in the initiation and reinitiation of virus-specific RNA synthesis is discussed.
DOI：
10.1073/pnas.83.17.6282
发表时间：
1986-09-01
影响因子：
11.1
作者：
BEATON, AR;KRUG, RM
通讯作者：
KRUG, RM
DOI：
10.1073/pnas.78.12.7355
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ULMANEN, I;BRONI, BA;KRUG, RM
通讯作者：
KRUG, RM