PHYSIOLOGICAL REGULATION OF ACETYL-COA CARBOXYLASE GENE-EXPRESSION - EFFECTS OF DIET, DIABETES, AND LACTATION ON ACETYL-COA CARBOXYLASE MESSENGER-RNA
PHYSIOLOGICAL REGULATION OF ACETYL-COA CARBOXYLASE GENE-EXPRESSION - EFFECTS OF DIET, DIABETES, AND LACTATION ON ACETYL-COA CARBOXYLASE MESSENGER-RNA
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DOI：
10.1016/0003-9861(88)90013-6
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发表时间：
1988-11-15
影响因子：
3.9
通讯作者：
KIM, KH
中科院分区：
文献类型：
--
作者：
PAPE, ME;LOPEZCASILLAS, F;KIM, KH
我们使用 cDNA 探针测量了不同营养和激素状态下大鼠各组织中乙酰辅酶 A 羧化酶 mRNA 水平。我们调查了已知会改变羧化酶活性从而改变脂肪酸合成的生理条件，以确定是否涉及羧化酶 mRNA 水平的变化。目前的研究包括禁食和重新进食、糖尿病和胰岛素以及哺乳对羧化酶 mRNA 水平的影响。肝脏 RNA 的 Northern 印迹分析表明，与禁食条件相比，禁食后再给动物重新喂食无脂肪（高碳水化合物）饮食，羧化酶 mRNA 的量显着增加。 mRNA 水平的这些变化对应于乙酰辅酶A羧化酶的活性和量的变化。附睾脂肪组织中的乙酰辅酶A羧化酶 mRNA 水平在禁食时下降，并在重新进食 72 小时后增加至几乎正常水平，与肝脏反应非常相似。与非糖尿病动物相比，糖尿病大鼠附睾脂肪组织中乙酰辅酶A羧化酶mRNA的量显着减少。然而，注射胰岛素 6 小时后，mRNA 水平恢复到非糖尿病动物的水平。妊娠和哺乳期也影响肝脏和乳腺中羧化酶 mRNA 的水平。两种组织的最大诱导发生在产后 5 天。这些研究表明，这些不同的生理条件部分通过改变乙酰 CaA 羧化酶基因表达来影响脂肪酸合成。
We measured acetyl-CoA carboxylase mRNA levels in various tissues of the rat under different nutritional and hormonal states using a cDNA probe. We surveyed physiological conditions which are known to alter carboxylase activity, and thus fatty acid synthesis, to determine whether changes in the levels of carboxylase mRNA are involved. The present studies include the effects of fasting and refeeding, diabetes and insulin, and lactation on carboxylase mRNA levels. Northern blot analysis of liver RNA revealed that fasting followed by refeeding animals a fat-free (high carbohydrate) diet dramatically increased the amount of carboxylase mRNA compared to the fasted condition. These changes in the level of mRNA correspond to changes in the activity and amount of acetyl-CoA carboxylase. Acetyl-CoA carboxylase mRNA levels in epididymal fat tissue decreased upon fasting and increased to virtually normal levels after 72 h of refeeding, closely resembling the liver response. The amount of acetyl-CoA carboxylase mRNA decreased markedly in epididymal fat tissue of diabetic rats as compared to nondiabetic animals. However, 6 h after injection of insulin the mRNA level returned to that of the nondiabetic animals. Gestation and lactation also affected the levels of carboxylase mRNA in both liver and mammary gland. Maximum induction in both tissues occurred 5 days postpartum. These studies suggest that these diverse physiological conditions affect fatty acid synthesis in part by altering acetyl-CaA carboxylase gene expression.